Chromatographic behaviour and purification of linear lambda phage and plasmid DNA molecules on 2-hydroxyethyl methacrylate-ethylene dimethacrylate-based supports

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F03%3A00008144" target="_blank" >RIV/00216224:14310/03:00008144 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Chromatographic behaviour and purification of linear lambda phage and plasmid DNA molecules on 2-hydroxyethyl methacrylate-ethylene dimethacrylate-based supports

Popis výsledku v původním jazyce

The HEMA-BIO 1000 supports based on a copolymer of 2-hydroxyethyl methacrylate and ethylene dimethacrylate were used for separation of lambda DNA and its fragments and plasmid pBR322 DNA. The separation of fragments greater than 6.6 kbp was demonstratedaccording to the slalom chromatography mechanism on column for gel permeation chromatography in the case of linear lambda DNA fragments. The influence of the particle size of column packing, mobile phase rate, and KCl concentration in mobile phase was discussed. The purification of plasmid DNA pBR322 using GPC was more rapid in comparison with gel electrophoresis. The presence of salts in the eluate is not disadvantageous. DNA can be recovered from the eluate by ethanol precipitation. Plasmid DNA pBR322isolated in this way was suitable for different biological applications (cleavage with restrictases, electrotransformation into bacterial cells).

Název v anglickém jazyce

Chromatographic behaviour and purification of linear lambda phage and plasmid DNA molecules on 2-hydroxyethyl methacrylate-ethylene dimethacrylate-based supports

Popis výsledku anglicky

The HEMA-BIO 1000 supports based on a copolymer of 2-hydroxyethyl methacrylate and ethylene dimethacrylate were used for separation of lambda DNA and its fragments and plasmid pBR322 DNA. The separation of fragments greater than 6.6 kbp was demonstratedaccording to the slalom chromatography mechanism on column for gel permeation chromatography in the case of linear lambda DNA fragments. The influence of the particle size of column packing, mobile phase rate, and KCl concentration in mobile phase was discussed. The purification of plasmid DNA pBR322 using GPC was more rapid in comparison with gel electrophoresis. The presence of salts in the eluate is not disadvantageous. DNA can be recovered from the eluate by ethanol precipitation. Plasmid DNA pBR322isolated in this way was suitable for different biological applications (cleavage with restrictases, electrotransformation into bacterial cells).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F98%2F1231" target="_blank" >GA203/98/1231: Orientovaná imobilizace enzymů a protilátek s využitím biospecifických interakcí</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Svazek periodika

1009

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

207-214

Kód UT WoS článku

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EID výsledku v databázi Scopus

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