Substratová specifita haloalkán dehalogenáz.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00009966" target="_blank" >RIV/00216224:14310/04:00009966 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Substrate specificity of haloalkane dehalogenases

Popis výsledku v původním jazyce

Haloalkane dehalogenases are microbial enzymes capable to cleave carbon-halogen bond in halogenated aliphatic compounds. There is a growing interest in these enzymes because of their potential use in bioremediation, as industrial biocatalysts, or as biosensors. Structurally, haloalkane dehalogenases belong to the a/b-hydrolase fold superfamily. Best studied haloalkane dehalogeneses are DhlA from Xanthobacter autotrophicus GJ10 [1], DhaA from Rhodococcus rhodochrous NCIMB 13064 [2] and LinB from Sphingomonas paucimobilis UT26 [3]. Crystal structures of these haloalkane dehalogenases are known. There is a number of DNA sequences which are expected to code haloalkane dehalogenases. This expectation is based on sequential similiraties with known haloalkanedehalogenases: DhlA, DhaA and LinB. Protein Rv2579 from Mycobacterium tuberculosis H37Rv has 68 % sequence similarity with LinB. Gene rv2579 was cloned to Escherichia coli, protein Rv2579 was overexpressed and purified to homogeneity. Ha

Název v anglickém jazyce

Substrate specificity of haloalkane dehalogenases

Popis výsledku anglicky

Haloalkane dehalogenases are microbial enzymes capable to cleave carbon-halogen bond in halogenated aliphatic compounds. There is a growing interest in these enzymes because of their potential use in bioremediation, as industrial biocatalysts, or as biosensors. Structurally, haloalkane dehalogenases belong to the a/b-hydrolase fold superfamily. Best studied haloalkane dehalogeneses are DhlA from Xanthobacter autotrophicus GJ10 [1], DhaA from Rhodococcus rhodochrous NCIMB 13064 [2] and LinB from Sphingomonas paucimobilis UT26 [3]. Crystal structures of these haloalkane dehalogenases are known. There is a number of DNA sequences which are expected to code haloalkane dehalogenases. This expectation is based on sequential similiraties with known haloalkanedehalogenases: DhlA, DhaA and LinB. Protein Rv2579 from Mycobacterium tuberculosis H37Rv has 68 % sequence similarity with LinB. Gene rv2579 was cloned to Escherichia coli, protein Rv2579 was overexpressed and purified to homogeneity. Ha

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/LN00A016" target="_blank" >LN00A016: BIOMOLEKULÁRNÍ CENTRUM</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Materials Structure in Chemistry, Biology, Physics and Technology

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

32-33

Název nakladatele

Czech and Slovak Crystallographic Association

Místo vydání

Praha

Místo konání akce

Univerzitní a akademické centrum Nové Hrady

Datum konání akce

11. 3. 2004

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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