Studium inhibice enzymů kombinací metody EMMA s technikou částečného plnění kapiláry a přímou či nepřímou detekcí.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00009987" target="_blank" >RIV/00216224:14310/04:00009987 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Electrophoretically mediated microanalysis with partial filling technique and indirect or direct detection as a tool for inhibition studies of enzymatic reactions

Popis výsledku v původním jazyce

The inhibition study of the model enzyme haloalkane dehalogenase from Sphingomonas paucimobilis was performed by a combination of the electrophoretically mediated microanalysis with a partial filling technique and indirect or direct detection. In this set-up, the part of capillary is filled with the buffer suitable for the enzymatic reaction 20 mM glycine buffer (pH 8.6) whereas, the rest of the capillary is filled with the background electrolyte optimal for separation of substrates and products. Two different background electrolytes and corresponding detection approaches were used to show the versatility of the developed method. The inhibition effect of 1,2-dichloroethane on the dehalogenation of brominated substrate 1-bromobutane was studied by meansof 10 mM chromate - 0.1 mM cetyltrimethylammonium bromide (pH 9.2) in combination with an indirect detection or 0.1 M beta-alanine - hydrochloric acid (pH 3.5) in combination with a direct detection. The method was used not only to estim

Název v anglickém jazyce

Electrophoretically mediated microanalysis with partial filling technique and indirect or direct detection as a tool for inhibition studies of enzymatic reactions

Popis výsledku anglicky

The inhibition study of the model enzyme haloalkane dehalogenase from Sphingomonas paucimobilis was performed by a combination of the electrophoretically mediated microanalysis with a partial filling technique and indirect or direct detection. In this set-up, the part of capillary is filled with the buffer suitable for the enzymatic reaction 20 mM glycine buffer (pH 8.6) whereas, the rest of the capillary is filled with the background electrolyte optimal for separation of substrates and products. Two different background electrolytes and corresponding detection approaches were used to show the versatility of the developed method. The inhibition effect of 1,2-dichloroethane on the dehalogenation of brominated substrate 1-bromobutane was studied by meansof 10 mM chromate - 0.1 mM cetyltrimethylammonium bromide (pH 9.2) in combination with an indirect detection or 0.1 M beta-alanine - hydrochloric acid (pH 3.5) in combination with a direct detection. The method was used not only to estim

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

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Svazek periodika

24

Číslo periodika v rámci svazku

7-8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

1028

Kód UT WoS článku

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EID výsledku v databázi Scopus

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