TISSUE SPECIFIC ALTERNATIVE SPLICING IN THE GENE FOR AHP5, A HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN FROM ARABIDOPSIS THALIANA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00010100" target="_blank" >RIV/00216224:14310/04:00010100 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

čeština

Název v původním jazyce

TISSUE SPECIFIC ALTERNATIVE SPLICING IN THE GENE FOR AHP5, A HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN FROM ARABIDOPSIS THALIANA

Popis výsledku v původním jazyce

A multistep two-component system is involved in signal transduction in Arabidopsis and consists of a sensory histidine kinase, a phosphotransfer factor and a response regulator. Factors with histidine-containing phosphotransfer domains (AHPs) transfer the phosphoryl group from membrane localised receptor (histidine kinases) to effectors (response regulators) localised in the nucleus. Five AHPs have been identified in Arabidopsis. AHPs are small proteins (14,5 18 kDa), with a predicted acidic isoeletricpoint (5.53.9) and a typical phosphotransfer domain, including the conserved sequence XHQXKGSSXS. AHP5 comprises six exons and five introns. RNA was prepared using Trizol reagent from different plant tissues: leaves, roots, shoot, flowers, siliques and seedlings. Using RT-PCR we detected two products (AHP5s and AHP5l). Following sequencing, the second intron was found intact at position 211 bp in the longer product (AHP5l). Real time RT-PCR showed that the ratio between spliced and nonsp

Název v anglickém jazyce

TISSUE SPECIFIC ALTERNATIVE SPLICING IN THE GENE FOR AHP5, A HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN FROM ARABIDOPSIS THALIANA

Popis výsledku anglicky

A multistep two-component system is involved in signal transduction in Arabidopsis and consists of a sensory histidine kinase, a phosphotransfer factor and a response regulator. Factors with histidine-containing phosphotransfer domains (AHPs) transfer the phosphoryl group from membrane localised receptor (histidine kinases) to effectors (response regulators) localised in the nucleus. Five AHPs have been identified in Arabidopsis. AHPs are small proteins (14,5 18 kDa), with a predicted acidic isoeletricpoint (5.53.9) and a typical phosphotransfer domain, including the conserved sequence XHQXKGSSXS. AHP5 comprises six exons and five introns. RNA was prepared using Trizol reagent from different plant tissues: leaves, roots, shoot, flowers, siliques and seedlings. Using RT-PCR we detected two products (AHP5s and AHP5l). Following sequencing, the second intron was found intact at position 211 bp in the longer product (AHP5l). Real time RT-PCR showed that the ratio between spliced and nonsp

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

VIII.Pracovní setkání biochemik a molekulárních biolog

ISBN

80-210-3321-5

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

25-26

Název nakladatele

Masarykova univerzita v Brn

Místo vydání

Brno

Místo konání akce

Konferenní centrum USKM Vinaská v Brn

Datum konání akce

3. 2. 2004

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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