Charakterizace genomu a proteomu polyvalentního fága 812

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00010413" target="_blank" >RIV/00216224:14310/04:00010413 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Genomic and proteomic characterization of the polyvalent staphylophage 812

Popis výsledku v původním jazyce

Virulent and polyvalent staphylophage 812, a member of Myoviridae family, exhibits strong lytic activity on a broad host range of Staphylococcus species, thus being a suitable candidate for treatment of staphylococcal infections. Genome of this phage isrepresented by linear 145 kb dsDNA. Our study was focused on genome and proteome comparison of 812 wild-type phage, its host-range mutants and related phages U16, 131 and SK311 with the aim to explain molecular basis of differences in their host-range. The genomes of phages under study were compared by DNA restriction analysis, and the genomic regions showing differences in individual phages were characterized in details. The proteome analysis included separation of phage protein lysates by 1D SDS-PAGEwith subsequent identification of proteins by peptide mapping using MALDI-TOF mass spectrometry. Comparison of the phage genomes revealed several rearrangements of DNA sequences as well as single nucleotide substitutions in different part

Název v anglickém jazyce

Genomic and proteomic characterization of the polyvalent staphylophage 812

Popis výsledku anglicky

Virulent and polyvalent staphylophage 812, a member of Myoviridae family, exhibits strong lytic activity on a broad host range of Staphylococcus species, thus being a suitable candidate for treatment of staphylococcal infections. Genome of this phage isrepresented by linear 145 kb dsDNA. Our study was focused on genome and proteome comparison of 812 wild-type phage, its host-range mutants and related phages U16, 131 and SK311 with the aim to explain molecular basis of differences in their host-range. The genomes of phages under study were compared by DNA restriction analysis, and the genomic regions showing differences in individual phages were characterized in details. The proteome analysis included separation of phage protein lysates by 1D SDS-PAGEwith subsequent identification of proteins by peptide mapping using MALDI-TOF mass spectrometry. Comparison of the phage genomes revealed several rearrangements of DNA sequences as well as single nucleotide substitutions in different part

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F03%2F0515" target="_blank" >GA203/03/0515: Integrovaná analýza genomu a proteomu terapeuticky významných bakteriofágů kombinací elektroforézy a hmotnostní spektrometrie</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

11th International Symposium on Staphylococci & Staphylococcal Infections. Plenary Summaries & Poster Abstracts

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

201

Název nakladatele

Medical University of South Carolina

Místo vydání

Charleston, South Carolina, USA

Místo konání akce

Charleston, South Carolina, USA

Datum konání akce

24. 10. 2004

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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