Expressional regulation of CKI1 provides molecular evidence for hormone interplay during Arabidopsis root development

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00013449" target="_blank" >RIV/00216224:14310/05:00013449 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Expressional regulation of CKI1 provides molecular evidence for hormone interplay during Arabidopsis root development

Popis výsledku v původním jazyce

Gametophytic expression of sensor histidine kinase CKI1, originally implicated in cytokinin signaling, was shown to be essential for megagametogenesis in Arabidopsis. Using CKI1 promoter::uidA lines and CKI1 mRNA and protein in situ localization, we haveidentified the activity of CKI1 in both root (root tip, vascular tissue and lateral root primordia) and shoot (shoot apical meristem, vascular tissue of cotyledons, floral organs, flower pedicels and inflorescence stem). We have found that the activityof CKI1 promoter in the root (but not the shoot) of young seedlings could be manipulated by exogenous application of cytokinin, auxin and auxin transport inhibitors. The ectopic expression domain of CKI1 after application of both hormones overlaps with auxin intracellular local maxima as identified by the auxin responsive promoter DR5.

Název v anglickém jazyce

Expressional regulation of CKI1 provides molecular evidence for hormone interplay during Arabidopsis root development

Popis výsledku anglicky

Gametophytic expression of sensor histidine kinase CKI1, originally implicated in cytokinin signaling, was shown to be essential for megagametogenesis in Arabidopsis. Using CKI1 promoter::uidA lines and CKI1 mRNA and protein in situ localization, we haveidentified the activity of CKI1 in both root (root tip, vascular tissue and lateral root primordia) and shoot (shoot apical meristem, vascular tissue of cotyledons, floral organs, flower pedicels and inflorescence stem). We have found that the activityof CKI1 promoter in the root (but not the shoot) of young seedlings could be manipulated by exogenous application of cytokinin, auxin and auxin transport inhibitors. The ectopic expression domain of CKI1 after application of both hormones overlaps with auxin intracellular local maxima as identified by the auxin responsive promoter DR5.

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů