The yield of chromosomal aberrations in children with acute leukemia using methods GTG banding, FISH, CGH and HR-CGH

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00014618" target="_blank" >RIV/00216224:14310/05:00014618 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The yield of chromosomal aberrations in children with acute leukemia using methods GTG banding, FISH, CGH and HR-CGH

Popis výsledku v původním jazyce

We report results from the study of 33 children with acute lymphoid (25) and myeloid (8) leukaemia. Karyotyping of bone marrow cells is an important step for the precise diagnosis and for the choice of the adequate treatment in leukaemic patients. Twentysix patients were examined in the time of diagnosis and seven in relapse. The cytogenetic analyses were performed on 24-hours bone marrow cells cultured in RPMI 1640 according to the standard methods. GTG banding technique revealed clonal karyotypic abnormalities in 45% (15) samples. Cytogenetic findings were confirmed by fluorescence in situ hybridization (FISH) method. Comparative genomic hybridization (CGH) was used on bone marrow samples obtained from this patients in time of diagnosis or relapse.CGH showed new DNA copy number changes in 70% (23) patients. For the first time we were tested the modification of CGH. We show examples of submicroscopic chromosomal imbalances being detected with this high resolution CGH technique (HR-C

Název v anglickém jazyce

The yield of chromosomal aberrations in children with acute leukemia using methods GTG banding, FISH, CGH and HR-CGH

Popis výsledku anglicky

We report results from the study of 33 children with acute lymphoid (25) and myeloid (8) leukaemia. Karyotyping of bone marrow cells is an important step for the precise diagnosis and for the choice of the adequate treatment in leukaemic patients. Twentysix patients were examined in the time of diagnosis and seven in relapse. The cytogenetic analyses were performed on 24-hours bone marrow cells cultured in RPMI 1640 according to the standard methods. GTG banding technique revealed clonal karyotypic abnormalities in 45% (15) samples. Cytogenetic findings were confirmed by fluorescence in situ hybridization (FISH) method. Comparative genomic hybridization (CGH) was used on bone marrow samples obtained from this patients in time of diagnosis or relapse.CGH showed new DNA copy number changes in 70% (23) patients. For the first time we were tested the modification of CGH. We show examples of submicroscopic chromosomal imbalances being detected with this high resolution CGH technique (HR-C

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

European Journal of Human Genetics

ISBN

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ISSN

1476-5438

e-ISSN

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Počet stran výsledku

1

Strana od-do

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Název nakladatele

Nature Publishing Group

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

1. 1. 2005

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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