Izolace a charakterizace nového mutanta v genu pro protein obsahující PPR motiv u Arabidopsis thaliana

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00021384" target="_blank" >RIV/00216224:14310/05:00021384 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein

Popis výsledku v původním jazyce

A novel Arabidopsis thaliana mutant of one member of the PPR (pentatricopeptide repeat) gene family has been identified during the screening of 2500 Arabidopsis individual insertion T2 lines. The deviations in growth and development of the mutant plantsappeared already at the initial growth. The germination was completely inhibited (about 30 to 40% of the T3 seeds in each line) or distinctly delayed in comparison with wt plants. The next development of the mutant seedlings varied further on agar medium. Complex phenotypic expression from post-embryonic lethality associated with abnormal pattern of cell division during globular to heart transition to fertile plants with just subtle phenotypic changes was observed within this individual mutant line. TheSouthern blot and segregation analysis revealed that the mutant plants contained one insert of T-DNA tightly linked to the mutant locus. To be sure that the ambiguous mutant phenotype was due to a mutation at a single locus, we have mapp

Název v anglickém jazyce

Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein

Popis výsledku anglicky

A novel Arabidopsis thaliana mutant of one member of the PPR (pentatricopeptide repeat) gene family has been identified during the screening of 2500 Arabidopsis individual insertion T2 lines. The deviations in growth and development of the mutant plantsappeared already at the initial growth. The germination was completely inhibited (about 30 to 40% of the T3 seeds in each line) or distinctly delayed in comparison with wt plants. The next development of the mutant seedlings varied further on agar medium. Complex phenotypic expression from post-embryonic lethality associated with abnormal pattern of cell division during globular to heart transition to fertile plants with just subtle phenotypic changes was observed within this individual mutant line. TheSouthern blot and segregation analysis revealed that the mutant plants contained one insert of T-DNA tightly linked to the mutant locus. To be sure that the ambiguous mutant phenotype was due to a mutation at a single locus, we have mapp

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Book of Abstracts, 6th International Symposium in the Series Recent Advances in Plant Biotechnology, 12.-16.9.2005

ISBN

80-86778-16-9

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

46

Název nakladatele

Institute of Plant Molecular Biology ASCR

Místo vydání

České Budějovice

Místo konání akce

České Budějovice

Datum konání akce

1. 1. 2005

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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