Ovlivnění redukce dusičnanu v nerostoucích buňkách Paracoccus denitrificans chlorečnanem a chloritanem

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00017489" target="_blank" >RIV/00216224:14310/06:00017489 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Interference of chlorate and chlorite with nitrate reduction in resting cells of Paracoccus denitrificans

Popis výsledku v původním jazyce

When grown anaerobically on a succinate-nitrate medium (SN), Paracoccus denitrificans forms the membrane-bound, cytoplasmically-oriented, chlorate-reducing nitrate reductase Nar, while the periplasmic enzyme Nap is expressed during the aerobic growth onbutyrate (BO). Preincubation of SN cells with chlorate produced a concentration-dependent decrease in nitrate utilization, which could be ascribed to Nar inactivation. Toluenisation rendered Nar less sensitive to chlorate, but more sensitive to chlorite.Nap enzyme of BO cells was inactivated by both chlorate and chlorite at concentrations that were at least two orders of magnitude lower than those shown to affect Nar. Partial purification of Nap resulted in an insensitivity to chlorate and a diminishedsensitivity to chlorite. Azide was specific for SN cells in protecting nitrate reductase against chlorate attack, the protective effect of nitrate being more pronounced in BO cells. The results are discussed in terms of different metabol

Název v anglickém jazyce

Interference of chlorate and chlorite with nitrate reduction in resting cells of Paracoccus denitrificans

Popis výsledku anglicky

When grown anaerobically on a succinate-nitrate medium (SN), Paracoccus denitrificans forms the membrane-bound, cytoplasmically-oriented, chlorate-reducing nitrate reductase Nar, while the periplasmic enzyme Nap is expressed during the aerobic growth onbutyrate (BO). Preincubation of SN cells with chlorate produced a concentration-dependent decrease in nitrate utilization, which could be ascribed to Nar inactivation. Toluenisation rendered Nar less sensitive to chlorate, but more sensitive to chlorite.Nap enzyme of BO cells was inactivated by both chlorate and chlorite at concentrations that were at least two orders of magnitude lower than those shown to affect Nar. Partial purification of Nap resulted in an insensitivity to chlorate and a diminishedsensitivity to chlorite. Azide was specific for SN cells in protecting nitrate reductase against chlorate attack, the protective effect of nitrate being more pronounced in BO cells. The results are discussed in terms of different metabol

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microbiology-SGM

ISSN

1350-0872

e-ISSN

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Svazek periodika

152

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

3529-3534

Kód UT WoS článku

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EID výsledku v databázi Scopus

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