Characterization of staphylococcal type strains by whole-cell protein analysis and (GTG)5-PCR fingerprinting.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00021871" target="_blank" >RIV/00216224:14310/06:00021871 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of staphylococcal type strains by whole-cell protein analysis and (GTG)5-PCR fingerprinting.

Popis výsledku v původním jazyce

A group of 47 type strains representing all validly described staphylococcal species and subspecies was characterized by whole-cell protein analysis by using sodium dodecylsulfate-polyacrylamide gel electrophoresis and rep-PCR fingerprinting with the (GTG)5 primer. All strains were well typeable and revealed unique fingerprints by both methods. The individual staphylococcal type strains were differentiated on the level of 74 % using the protein analysis, with exception of novobiocin-resistant species, and on the level of 68 % by using the (GTG)5-PCR fingerprinting. Generally, all subspecies of the individual species showed close protein profiles that were grouped into individual clusters. Similarly, but with a few exceptions, the individual subspecieswere separated into the distinct clusters using the (GTG)5-PCR fingerprinting. No clear correlation between the clustering obtained by both methods and the phylogenetic relationships of the analysed species was observed.

Název v anglickém jazyce

Characterization of staphylococcal type strains by whole-cell protein analysis and (GTG)5-PCR fingerprinting.

Popis výsledku anglicky

A group of 47 type strains representing all validly described staphylococcal species and subspecies was characterized by whole-cell protein analysis by using sodium dodecylsulfate-polyacrylamide gel electrophoresis and rep-PCR fingerprinting with the (GTG)5 primer. All strains were well typeable and revealed unique fingerprints by both methods. The individual staphylococcal type strains were differentiated on the level of 74 % using the protein analysis, with exception of novobiocin-resistant species, and on the level of 68 % by using the (GTG)5-PCR fingerprinting. Generally, all subspecies of the individual species showed close protein profiles that were grouped into individual clusters. Similarly, but with a few exceptions, the individual subspecieswere separated into the distinct clusters using the (GTG)5-PCR fingerprinting. No clear correlation between the clustering obtained by both methods and the phylogenetic relationships of the analysed species was observed.

Druh

O - Ostatní výsledky

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů