Assessment of retinoic activity in fly ash and air samples from polluted areas using P19/A15 cell line

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00020343" target="_blank" >RIV/00216224:14310/07:00020343 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Assessment of retinoic activity in fly ash and air samples from polluted areas using P19/A15 cell line

Popis výsledku v původním jazyce

Contamination in the environment has been described to induce malformations in amphibians. This fact could be linked with anti/retinoic action of the contaminants because retinoids control morphogenesis by affecting processes such as differentiation or apoptosis. In vitro model for evaluation of anti/retinoic action of xenobiotics was developed. It is based on murine cell line P19, which retains a pluripotent character and its differentiation is regulated mainly by retinoids. In the derived cell line P19/A15, the retinoic response element was linked with luciferase gene enabling the study of retinoid signaling. We tested air samples extracts from two areas in the vicinity of chemical factories producing different types of pollutants, extracts from flyash from municipal incinerator and reference sample from background locality. Important activities were observed in both gas and particulate fractions of multiple air samples and in fly ash samples.

Název v anglickém jazyce

Assessment of retinoic activity in fly ash and air samples from polluted areas using P19/A15 cell line

Popis výsledku anglicky

Contamination in the environment has been described to induce malformations in amphibians. This fact could be linked with anti/retinoic action of the contaminants because retinoids control morphogenesis by affecting processes such as differentiation or apoptosis. In vitro model for evaluation of anti/retinoic action of xenobiotics was developed. It is based on murine cell line P19, which retains a pluripotent character and its differentiation is regulated mainly by retinoids. In the derived cell line P19/A15, the retinoic response element was linked with luciferase gene enabling the study of retinoid signaling. We tested air samples extracts from two areas in the vicinity of chemical factories producing different types of pollutants, extracts from flyash from municipal incinerator and reference sample from background locality. Important activities were observed in both gas and particulate fractions of multiple air samples and in fly ash samples.

Druh

O - Ostatní výsledky

CEP obor

DN - Vliv životního prostředí na zdraví

OECD FORD obor

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Projekt

<a href="/cs/project/GP525%2F05%2FP160" target="_blank" >GP525/05/P160: In vitro modely pro studium endokrinní disrupce - účinky tradičních a nových typů persistentních organických polutantů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů