Characterization of Lactococcus lactis subsp. lactis isolated from surface waters
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00022761" target="_blank" >RIV/00216224:14310/07:00022761 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Characterization of Lactococcus lactis subsp. lactis isolated from surface waters
Popis výsledku v původním jazyce
A group of nine strains isolated on Slanetz-Bartley agar and/or Kanamycin esculin azide agar were isolated as presumptive enterococci during a screening of Enterococcus spp. in surface waters. All analysed strains were clearly separated using rep-PCR with the (GTG)5 primer from all enterococci included in the in-house CCM database but they formed a homogeneous cluster matching Lactococcus lactis subsp. lactis representatives. Further identification using extensive biotyping as well as automated ribotyping with EcoRI (RiboPrinter microbial characterization system) confirmed all strains as L. lactis subsp. lactis. Obtained results showed that L. lactis subsp. lactis can be commonly isolated from different surface waters. These strains can be confused with enterococci due to their good growth on enterococcal selective media and their similarity in key phenotypical tests. (GTG)5-PCR revealed as a reliable and fast method for identification of L. lactis subsp.
Název v anglickém jazyce
Characterization of Lactococcus lactis subsp. lactis isolated from surface waters
Popis výsledku anglicky
A group of nine strains isolated on Slanetz-Bartley agar and/or Kanamycin esculin azide agar were isolated as presumptive enterococci during a screening of Enterococcus spp. in surface waters. All analysed strains were clearly separated using rep-PCR with the (GTG)5 primer from all enterococci included in the in-house CCM database but they formed a homogeneous cluster matching Lactococcus lactis subsp. lactis representatives. Further identification using extensive biotyping as well as automated ribotyping with EcoRI (RiboPrinter microbial characterization system) confirmed all strains as L. lactis subsp. lactis. Obtained results showed that L. lactis subsp. lactis can be commonly isolated from different surface waters. These strains can be confused with enterococci due to their good growth on enterococcal selective media and their similarity in key phenotypical tests. (GTG)5-PCR revealed as a reliable and fast method for identification of L. lactis subsp.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2007
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů