Předpověď retenčních časů tryptických peptidů pomocí tzv. měkkého modelování jako nástroj pro proteomiku vedenou kapalinovou chromatografií a hmotnostní spektrometrií

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00026284" target="_blank" >RIV/00216224:14310/07:00026284 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Prediction of tryptic peptide retention times by means of soft modelling as a tool for liquid chromatography-mass spectrometry driven proteomics

Popis výsledku v původním jazyce

To predict a retention time of analyte is already thoroughly researched topic. The knowledge of retention time value could serve many purposes, in LC-MS driven proteomics to know retention time of peptide may help to deal with acquired mass spectrometricdata in order to maximise the information gain by means of number of identified peptides. Among the two principal approaches how to determine the retention time of compound, hard and soft modelling, the soft models have the advantage of being based on black box principle, what means the relations between respective retention time and compound properties in combination with separation system properties can be found without any exact knowledge of physico-chemical relation between them. The proper choiceof compound descriptors and relevant settings of separation system may result in precise determination of retention time. As for the proteomics application, once it is possible to make a link between tryptic peptide sequence and its reten

Název v anglickém jazyce

Prediction of tryptic peptide retention times by means of soft modelling as a tool for liquid chromatography-mass spectrometry driven proteomics

Popis výsledku anglicky

To predict a retention time of analyte is already thoroughly researched topic. The knowledge of retention time value could serve many purposes, in LC-MS driven proteomics to know retention time of peptide may help to deal with acquired mass spectrometricdata in order to maximise the information gain by means of number of identified peptides. Among the two principal approaches how to determine the retention time of compound, hard and soft modelling, the soft models have the advantage of being based on black box principle, what means the relations between respective retention time and compound properties in combination with separation system properties can be found without any exact knowledge of physico-chemical relation between them. The proper choiceof compound descriptors and relevant settings of separation system may result in precise determination of retention time. As for the proteomics application, once it is possible to make a link between tryptic peptide sequence and its reten

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Trends in Chromatography

ISSN

0972-8635

e-ISSN

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Svazek periodika

3

Číslo periodika v rámci svazku

-

Stát vydavatele periodika

IN - Indická republika

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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