Studium inhibice cytochromů P450 pomocí MEKC

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00024585" target="_blank" >RIV/00216224:14310/08:00024585 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Application of Micellar Electrokinetic Capillary Chromatography for Evaluation of Inhibitory Effects on Cytochrome P450 Reaction

Popis výsledku v původním jazyce

The major aim of this work is to demonstrate the applicability of micellar electrokinetic capillary chromatography with SDS based pseudostationary phase for the screening of cytochrome P450 inhibitors. In contrast with the other capillary electrophoresismodes the cytochrome P450 reaction mixture thus could be used for the analysis without any pre-treatment. Cytochrome P450 2C9, one of the most important isoforms in human liver, was chosen as a model example for this study in combination with diclofenacas a probe substrate. The inhibitory effect on the given cytochrome P450 reaction was evaluated for two inhibitors with different inhibition potential strong inhibitor sulfaphenazole and moderate inhibitor ketoconazole. As a result 50 % inhibitory concentrations IC50 and inhibition constants Ki were evaluated; their values for both inhibitors were in a good agreement with the literature data determined by different methods.

Název v anglickém jazyce

Application of Micellar Electrokinetic Capillary Chromatography for Evaluation of Inhibitory Effects on Cytochrome P450 Reaction

Popis výsledku anglicky

The major aim of this work is to demonstrate the applicability of micellar electrokinetic capillary chromatography with SDS based pseudostationary phase for the screening of cytochrome P450 inhibitors. In contrast with the other capillary electrophoresismodes the cytochrome P450 reaction mixture thus could be used for the analysis without any pre-treatment. Cytochrome P450 2C9, one of the most important isoforms in human liver, was chosen as a model example for this study in combination with diclofenacas a probe substrate. The inhibitory effect on the given cytochrome P450 reaction was evaluated for two inhibitors with different inhibition potential strong inhibitor sulfaphenazole and moderate inhibitor ketoconazole. As a result 50 % inhibitory concentrations IC50 and inhibition constants Ki were evaluated; their values for both inhibitors were in a good agreement with the literature data determined by different methods.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Svazek periodika

1189

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

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Kód UT WoS článku

000255698000025

EID výsledku v databázi Scopus

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