viz. angl.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00026345" target="_blank" >RIV/00216224:14310/08:00026345 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Sensitivity analysis of RecA genotoxicity test

Popis výsledku v původním jazyce

RecA test is a short-term luminescent SOS genotoxicity test. The induction of the SOS system is detected through the activation of the reporter gene system - DNA damage SOS repair system. The test is based on the swift elevation of recA gene expression in response to DNA damage. In case of RecA test there is a specific recA::luxCDABE gene fusion. It is fusion of reporter gene and specific gene of SOS repair system. This gene fusion could detect genotoxicity efficiently. In this study Escherichia coli strains DPD2794, DPD2797, DPD1718 and DPD1710 were used for detection of genotoxicity. The genotoxicity was detected by monitoring of bioluminescent emission. For assessment of the sensitivity evaluation a group of chemicals and environmental samples wereselected. The aim of this research was to decide whether this test has future applications in the screening evaluation of genotoxic potential and to compare sensitivity of the RecA test with various strains of Escherichia coli. Results in

Název v anglickém jazyce

Sensitivity analysis of RecA genotoxicity test

Popis výsledku anglicky

RecA test is a short-term luminescent SOS genotoxicity test. The induction of the SOS system is detected through the activation of the reporter gene system - DNA damage SOS repair system. The test is based on the swift elevation of recA gene expression in response to DNA damage. In case of RecA test there is a specific recA::luxCDABE gene fusion. It is fusion of reporter gene and specific gene of SOS repair system. This gene fusion could detect genotoxicity efficiently. In this study Escherichia coli strains DPD2794, DPD2797, DPD1718 and DPD1710 were used for detection of genotoxicity. The genotoxicity was detected by monitoring of bioluminescent emission. For assessment of the sensitivity evaluation a group of chemicals and environmental samples wereselected. The aim of this research was to decide whether this test has future applications in the screening evaluation of genotoxic potential and to compare sensitivity of the RecA test with various strains of Escherichia coli. Results in

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

DN - Vliv životního prostředí na zdraví

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Annals of Microbiology

ISSN

1590-4261

e-ISSN

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Svazek periodika

58

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

IT - Italská republika

Počet stran výsledku

5

Strana od-do

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Kód UT WoS článku

000254944600001

EID výsledku v databázi Scopus

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