Fotomorfogeneze indukovaná citokininy u Arabidopsis pěstované bez přístupu světla: Proteomická analýza

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00027268" target="_blank" >RIV/00216224:14310/08:00027268 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/08:00320007 RIV/61389030:_____/08:00320007 RIV/62156489:43210/08:00132733

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cytokinin-induced photomorphogenesis in dark-grown Arabidopsis: a proteomic analysis

Popis výsledku v původním jazyce

The effects on the proteome of increased levels of endogenous CKs in dark grown Arabidopsis seedlings were studied. Increase in endogenous CK levels was achieved by inducing the CK biosynthetic gene, ipt. Image and mass spectrometric analysis of proteinsseparated in two dimensional gels identified a number of differentially expressed proteins, most of which are related to chloroplast biogenesis and function. All the identified chloroplast proteins were upregulated in response to increased CK levels. Incontrast, all differentially regulated mitochondrial proteins, representing the second largest protein group, were downregulated. Comparison of our proteomic data with previously published transcriptome profiling of light induced photomorphogenesis andeffects of increased CK levels in light grown Arabidopsis seedlings revealed the same general trends for most of the 23 transcripts corresponding to the proteins in our data set.

Název v anglickém jazyce

Cytokinin-induced photomorphogenesis in dark-grown Arabidopsis: a proteomic analysis

Popis výsledku anglicky

The effects on the proteome of increased levels of endogenous CKs in dark grown Arabidopsis seedlings were studied. Increase in endogenous CK levels was achieved by inducing the CK biosynthetic gene, ipt. Image and mass spectrometric analysis of proteinsseparated in two dimensional gels identified a number of differentially expressed proteins, most of which are related to chloroplast biogenesis and function. All the identified chloroplast proteins were upregulated in response to increased CK levels. Incontrast, all differentially regulated mitochondrial proteins, representing the second largest protein group, were downregulated. Comparison of our proteomic data with previously published transcriptome profiling of light induced photomorphogenesis andeffects of increased CK levels in light grown Arabidopsis seedlings revealed the same general trends for most of the 23 transcripts corresponding to the proteins in our data set.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Experimental Botany

ISSN

0022-0957

e-ISSN

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Svazek periodika

59

Číslo periodika v rámci svazku

13

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

15

Strana od-do

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Kód UT WoS článku

000259973800018

EID výsledku v databázi Scopus

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