Staphylococcus sp. strains isolated from the common vole - the isolates of a new staphylococcal species

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00027319" target="_blank" >RIV/00216224:14310/08:00027319 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Staphylococcus sp. strains isolated from the common vole - the isolates of a new staphylococcal species

Popis výsledku v původním jazyce

Two strains of Gram-positive cocci isolated from viscera of common voles (Microtus arvalis) were studied by phenotyping and genotyping. Biochemical features were tested by Api Staph kits (bioMérieux) and by conventional tests. Detailed characterization was done by ribotyping, whole-cell protein analysis and rep-PCR using (GTG)5 primer. The phylogenetic position was analysed by partial 16S rRNA sequencing. Analysed strains revealed high mutual similarity of ribotype (EcoRI 88.9% and HindIII 78.6%), protein (95.6%) as well as rep-PCR (98.2%) fingerprints and concurrently were clearly distinct from all validly described staphylococcal taxa. The isolates showed the same partial 16S rRNA sequences, and the maximum sequence similarities were obtained with Staphylococcus muscae (98.3%) and Staphylococcus chromogenes (98.2%). The obtained results proved that the isolates are two different strains representing probably a novel staphylococcal species.

Název v anglickém jazyce

Staphylococcus sp. strains isolated from the common vole - the isolates of a new staphylococcal species

Popis výsledku anglicky

Two strains of Gram-positive cocci isolated from viscera of common voles (Microtus arvalis) were studied by phenotyping and genotyping. Biochemical features were tested by Api Staph kits (bioMérieux) and by conventional tests. Detailed characterization was done by ribotyping, whole-cell protein analysis and rep-PCR using (GTG)5 primer. The phylogenetic position was analysed by partial 16S rRNA sequencing. Analysed strains revealed high mutual similarity of ribotype (EcoRI 88.9% and HindIII 78.6%), protein (95.6%) as well as rep-PCR (98.2%) fingerprints and concurrently were clearly distinct from all validly described staphylococcal taxa. The isolates showed the same partial 16S rRNA sequences, and the maximum sequence similarities were obtained with Staphylococcus muscae (98.3%) and Staphylococcus chromogenes (98.2%). The obtained results proved that the isolates are two different strains representing probably a novel staphylococcal species.

Druh

O - Ostatní výsledky

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů