New CAPS Marker for Selection of a Barley Powdery Mildew Resistance Gene in the Mla Locus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00029161" target="_blank" >RIV/00216224:14310/09:00029161 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/25328859:_____/09:#0000423

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

New CAPS Marker for Selection of a Barley Powdery Mildew Resistance Gene in the Mla Locus

Popis výsledku v původním jazyce

In PI466495, a powdery mildew resistance source of wild barley (Hordeum vulgare ssp. spontaneum), one gene conferring powdery mildew resistance was identified in the Mla locus. In this paper, the RGH1a gene sequence was used as source for the developmentof a cleaved amplified polymorphic sequence (CAPS) marker. Cosegregation between this marker and powdery mildew resistance was analysed by specific DNA fragments associated with each allele of the gene using 286 F2 plants derived from a cross between winter barley (H. vulgare L.) variety Tiffany and PI466495. For the codominant marker RGH1aI1a, three fragments, 370 bp, 82 bp and 59 bp in size, were amplified from F2 plants exhibiting resistance reaction types 0 and 0/1 to powdery mildew; whereas two fragments, 429 bp and 82 bp in size, were amplified in susceptible plants. Simple procedures based on polymerase chain reaction and restriction enzyme digestion allowed for identifying the plants susceptible to powdery mildew (Blumeria gram

Název v anglickém jazyce

New CAPS Marker for Selection of a Barley Powdery Mildew Resistance Gene in the Mla Locus

Popis výsledku anglicky

In PI466495, a powdery mildew resistance source of wild barley (Hordeum vulgare ssp. spontaneum), one gene conferring powdery mildew resistance was identified in the Mla locus. In this paper, the RGH1a gene sequence was used as source for the developmentof a cleaved amplified polymorphic sequence (CAPS) marker. Cosegregation between this marker and powdery mildew resistance was analysed by specific DNA fragments associated with each allele of the gene using 286 F2 plants derived from a cross between winter barley (H. vulgare L.) variety Tiffany and PI466495. For the codominant marker RGH1aI1a, three fragments, 370 bp, 82 bp and 59 bp in size, were amplified from F2 plants exhibiting resistance reaction types 0 and 0/1 to powdery mildew; whereas two fragments, 429 bp and 82 bp in size, were amplified in susceptible plants. Simple procedures based on polymerase chain reaction and restriction enzyme digestion allowed for identifying the plants susceptible to powdery mildew (Blumeria gram

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F06%2F0608" target="_blank" >GA522/06/0608: Vývoj DNA markerů a konstrukce genetické mapy k lokalizaci nových genů odolnosti ječmene ozimého k padlí travnímu</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cereal Research Communications

ISSN

0133-3720

e-ISSN

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Svazek periodika

37

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

HU - Maďarsko

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000264755800011

EID výsledku v databázi Scopus

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