Utilization of cryogenically cooled ablation cell for GE of metalloproteins with LA ICP MS detection
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00029326" target="_blank" >RIV/00216224:14310/09:00029326 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Utilization of cryogenically cooled ablation cell for GE of metalloproteins with LA ICP MS detection
Popis výsledku v původním jazyce
Metalloproteins are responsible for many metabolic processes and gel electropohoresis (GE) is used to separate them. This study demonstrates use of cryogenic ablation cell for metalloprotein analysis by means of GE with LA-ICP-MS detection. After gel electrophoresis, proteins are detected by visualization in the gel. However this detection does not detect metal ions. The most complicated step of gel preparation for LA-ICP-MS analysis is drying. In case of utilization of cryogenic ablation cell, this time consuming step is removed. In this work, behaviour of gel in cryogenic cell at different temperature and other various conditions was studied.
Název v anglickém jazyce
Utilization of cryogenically cooled ablation cell for GE of metalloproteins with LA ICP MS detection
Popis výsledku anglicky
Metalloproteins are responsible for many metabolic processes and gel electropohoresis (GE) is used to separate them. This study demonstrates use of cryogenic ablation cell for metalloprotein analysis by means of GE with LA-ICP-MS detection. After gel electrophoresis, proteins are detected by visualization in the gel. However this detection does not detect metal ions. The most complicated step of gel preparation for LA-ICP-MS analysis is drying. In case of utilization of cryogenic ablation cell, this time consuming step is removed. In this work, behaviour of gel in cryogenic cell at different temperature and other various conditions was studied.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GA203%2F07%2F1391" target="_blank" >GA203/07/1391: Vývoj nových metod pro charakterizaci metaloproteinů</a><br>
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2009
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů