Substrate-Assited Laser Desorption Inductively-Coupled Plasma Mass Spectrometry for Elemental Detection in Microcolumn Effluent and Biological Submicroliter Samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00029473" target="_blank" >RIV/00216224:14310/09:00029473 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Substrate-Assited Laser Desorption Inductively-Coupled Plasma Mass Spectrometry for Elemental Detection in Microcolumn Effluent and Biological Submicroliter Samples

Popis výsledku v původním jazyce

A new method of sample preparation for elemental analysis using substrate-assisted laser desorption inductively-coupled plasma mass spectrometry (SALD ICP MS) is presented. Well-defined submicroliter volumes of analyzed samples are deposited onto polyethylene terephthalate glycol (PETG) plate and, after insertion into a commercial ablation cell, desorbed with pulses of 213-nm laser and analyzed with ICP MS. In contrast to conventional laser ablation (LA) ICP MS of solids, the analyzed compound is quantitatively desorbed during the PETG ablation. Furthermore, complete desorption is achieved at laser power density as low as ~10 MW/cm2, two orders below the levels typical in LA ICP MS. Model samples of metal salts in pg quantities were desorbed by less than 20 laser shots and relative spot-to-spot reproducibility was 3-10%. The limits of detection of Cr, Cu, Co, Fe, Ni, Sn and Zn deposited on the target were in the range of 50-500 fg.

Název v anglickém jazyce

Substrate-Assited Laser Desorption Inductively-Coupled Plasma Mass Spectrometry for Elemental Detection in Microcolumn Effluent and Biological Submicroliter Samples

Popis výsledku anglicky

A new method of sample preparation for elemental analysis using substrate-assisted laser desorption inductively-coupled plasma mass spectrometry (SALD ICP MS) is presented. Well-defined submicroliter volumes of analyzed samples are deposited onto polyethylene terephthalate glycol (PETG) plate and, after insertion into a commercial ablation cell, desorbed with pulses of 213-nm laser and analyzed with ICP MS. In contrast to conventional laser ablation (LA) ICP MS of solids, the analyzed compound is quantitatively desorbed during the PETG ablation. Furthermore, complete desorption is achieved at laser power density as low as ~10 MW/cm2, two orders below the levels typical in LA ICP MS. Model samples of metal salts in pg quantities were desorbed by less than 20 laser shots and relative spot-to-spot reproducibility was 3-10%. The limits of detection of Cr, Cu, Co, Fe, Ni, Sn and Zn deposited on the target were in the range of 50-500 fg.

Druh

O - Ostatní výsledky

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F09%2F1025" target="_blank" >GA203/09/1025: Multidetekční platforma pro mikrokolonové separace proteinů a peptidů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů