Quantitative proteomic analysis of lung carcinoma cell line transfected by mutant p53 gene sequences
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00029724" target="_blank" >RIV/00216224:14310/09:00029724 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Quantitative proteomic analysis of lung carcinoma cell line transfected by mutant p53 gene sequences
Popis výsledku v původním jazyce
The present study constitutes the use of a quantitative LC-MS/MS based proteomic approach for the comparative proteomic analysis of lung carcinoma cell line H1299 steady transfected by two different mutant p53 gene sequences (hot-spot mutations at codonpositions 175 and 273, respectively). The proteomics analysis method involved iTRAQ 8-plex stable isotope labeling of tryptic peptides, their separation via off-line coupled two-dimensional liquid chromatography (SCX and RP-LC) followed by tandem mass spectrometry on the MALDI-TOF/TOF platform (iTRAQ-2DLC-MS/MS). The principal aims of this study were: (1) to define the protein spectrum detectable using this approach, and (2) to quantitative compare the proteomic profiles of cells, which differ only in type of p53 mutation after 17-(allylamino)-17-demethoxygeldanamycin (17-AAG) treatment. The study resulted in the reproducible identification of 411 non-redundant proteins.
Název v anglickém jazyce
Quantitative proteomic analysis of lung carcinoma cell line transfected by mutant p53 gene sequences
Popis výsledku anglicky
The present study constitutes the use of a quantitative LC-MS/MS based proteomic approach for the comparative proteomic analysis of lung carcinoma cell line H1299 steady transfected by two different mutant p53 gene sequences (hot-spot mutations at codonpositions 175 and 273, respectively). The proteomics analysis method involved iTRAQ 8-plex stable isotope labeling of tryptic peptides, their separation via off-line coupled two-dimensional liquid chromatography (SCX and RP-LC) followed by tandem mass spectrometry on the MALDI-TOF/TOF platform (iTRAQ-2DLC-MS/MS). The principal aims of this study were: (1) to define the protein spectrum detectable using this approach, and (2) to quantitative compare the proteomic profiles of cells, which differ only in type of p53 mutation after 17-(allylamino)-17-demethoxygeldanamycin (17-AAG) treatment. The study resulted in the reproducible identification of 411 non-redundant proteins.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2009
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů