c-Myb expression at different stages of molar tooth germ

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00035656" target="_blank" >RIV/00216224:14310/09:00035656 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

c-Myb expression at different stages of molar tooth germ

Popis výsledku v původním jazyce

The aim of this work was to show c-myb expression profile in temporo-spatial model of mouse molar tooth germ development in the context of related tissues. Expression of c-myb (Abcam polyclonal antibody) was followed together with proliferation analysis(PCNAproliferating cell nuclear antigen, DAKO monoclonal antibody) and apoptosis detection (TUNEL test, terminal deoxynucleotidyl transferase mediated dUTP nick end labelling, Chemicon). Secondary biotinylated antibody was labelled by streptavidin-POD tovisualize positive cells by addition of DAB substrate. c-myb, PCNA and TUNEL were evaluated in the epithelial and mesenchymal parts of the tooth germ and in the surrounding interdental bone.

Název v anglickém jazyce

c-Myb expression at different stages of molar tooth germ

Popis výsledku anglicky

The aim of this work was to show c-myb expression profile in temporo-spatial model of mouse molar tooth germ development in the context of related tissues. Expression of c-myb (Abcam polyclonal antibody) was followed together with proliferation analysis(PCNAproliferating cell nuclear antigen, DAKO monoclonal antibody) and apoptosis detection (TUNEL test, terminal deoxynucleotidyl transferase mediated dUTP nick end labelling, Chemicon). Secondary biotinylated antibody was labelled by streptavidin-POD tovisualize positive cells by addition of DAB substrate. c-myb, PCNA and TUNEL were evaluated in the epithelial and mesenchymal parts of the tooth germ and in the surrounding interdental bone.

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů