PREPARATION AND MODIFICATION OF QUANTUM DOTS AS SELECTIVE PROBES IN BIOANALYSIS

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

PREPARATION AND MODIFICATION OF QUANTUM DOTS AS SELECTIVE PROBES IN BIOANALYSIS

Popis výsledku v původním jazyce

Quantum dots (QD) show a great potential as photoluminescent probes at cellular level, including in vivo immunolabeling or cellular tracking. The main advantages of quantum dots, when compared with the conventional organic fluorescent dyes, are practically no photobleaching, wide excitation and narrow emission spectra and size dependent emission wavelength. Typical materials for the preparation of quantum dots are elements of II - VI or III - V group. We have prepared CdTe water soluble quantum dots ofsizes 2 - 4,5 nm by the chemical reaction between CdCl2 and NaHTe at the presence of 3-mercaptopropionic acid. Thus, the photoluminescence emission maxima reach the values 520 - 700 nm. The emission spectra are narrow with the bandwidth of about 58 nm atthe half height of its intensity, while the excitation band lies in the range from 300 to 550 nm. There is a lot of simple conjugation techniques for linking of QD with biomolecules to use them as selective probes in immunolabeling.

Název v anglickém jazyce

PREPARATION AND MODIFICATION OF QUANTUM DOTS AS SELECTIVE PROBES IN BIOANALYSIS

Popis výsledku anglicky

Quantum dots (QD) show a great potential as photoluminescent probes at cellular level, including in vivo immunolabeling or cellular tracking. The main advantages of quantum dots, when compared with the conventional organic fluorescent dyes, are practically no photobleaching, wide excitation and narrow emission spectra and size dependent emission wavelength. Typical materials for the preparation of quantum dots are elements of II - VI or III - V group. We have prepared CdTe water soluble quantum dots ofsizes 2 - 4,5 nm by the chemical reaction between CdCl2 and NaHTe at the presence of 3-mercaptopropionic acid. Thus, the photoluminescence emission maxima reach the values 520 - 700 nm. The emission spectra are narrow with the bandwidth of about 58 nm atthe half height of its intensity, while the excitation band lies in the range from 300 to 550 nm. There is a lot of simple conjugation techniques for linking of QD with biomolecules to use them as selective probes in immunolabeling.

Druh

D - Stať ve sborníku

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

ChemZi 5/9 2009, 61. Zjazd chemikov, Vysoké Tatry, Slovensko

ISBN

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ISSN

1336-7242

e-ISSN

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Počet stran výsledku

1

Strana od-do

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Název nakladatele

Slovenská chemická spoločnost

Místo vydání

Bratislava

Místo konání akce

Vysoké Tatry

Datum konání akce

1. 1. 2009

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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