Testing of the influenza virus purification by capillary isoelectric focusing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00042757" target="_blank" >RIV/00216224:14310/10:00042757 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Testing of the influenza virus purification by capillary isoelectric focusing

Popis výsledku v původním jazyce

In the virological practice the preconcentration, purification and subsequently the determination of the purity and the concentration of the viruses from the cultural medium and/or from the real sample are required. The conventional techniques used todayare equipment demanding, time-consuming and labourious. In this study user friendly virus purification from the biological sample and the determination of the resulting viral sample purity by the capillary isoelectric focusing with UV detection are introduced. The Equine and Swine influenza viruses from the infected allantoic fluid from specific pathogen free embryonated chicken eggs were precipitaded by PEG 6000 and sodium chloride. The precipitated viruses were sedimented already at the accelerationabout 14 000 g. The impurities with different densities were removed on the sucrose gradients. The efficiency of the virus purification technique was examined by real-time PCR and compared with the results from the capillary isoelectric f

Název v anglickém jazyce

Testing of the influenza virus purification by capillary isoelectric focusing

Popis výsledku anglicky

In the virological practice the preconcentration, purification and subsequently the determination of the purity and the concentration of the viruses from the cultural medium and/or from the real sample are required. The conventional techniques used todayare equipment demanding, time-consuming and labourious. In this study user friendly virus purification from the biological sample and the determination of the resulting viral sample purity by the capillary isoelectric focusing with UV detection are introduced. The Equine and Swine influenza viruses from the infected allantoic fluid from specific pathogen free embryonated chicken eggs were precipitaded by PEG 6000 and sodium chloride. The precipitated viruses were sedimented already at the accelerationabout 14 000 g. The impurities with different densities were removed on the sucrose gradients. The efficiency of the virus purification technique was examined by real-time PCR and compared with the results from the capillary isoelectric f

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

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Svazek periodika

31/2010

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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