Antibacterial methods based on bioluminescent bacteria

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00049709" target="_blank" >RIV/00216224:14310/11:00049709 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Antibacterial methods based on bioluminescent bacteria

Popis výsledku v původním jazyce

Bioluminescent bacteria are common in salt water environment, but there is the only one genus of soil bacteria with natural bioluminescence ? Photorhabdus sp. These symbiotic bacteria reside the gut of entomopathogenic nematodes which are obligate insectparasites with the increasing importance as biological control agent. Isolated Photorhabdus luminescens was used separately for determination of pathogenity to insects. Except P. luminescens the artificial bioluminescent bacterium was used - geneticallymodified Escherichia coli K12 that carries Photorhabdus genes for bioluminescence. Both of these G- bacteria can be used for antibacterial assays based on their bioluminescence ability. Bioluminescence reaction is mediated by bacterial enzyme luciferasewhich catalyses the oxidation of long-chain aldehyde (substrate) and reduces flavin mononucleotide with emission of light. This emission can be immediately measured by the luminometer.

Název v anglickém jazyce

Antibacterial methods based on bioluminescent bacteria

Popis výsledku anglicky

Bioluminescent bacteria are common in salt water environment, but there is the only one genus of soil bacteria with natural bioluminescence ? Photorhabdus sp. These symbiotic bacteria reside the gut of entomopathogenic nematodes which are obligate insectparasites with the increasing importance as biological control agent. Isolated Photorhabdus luminescens was used separately for determination of pathogenity to insects. Except P. luminescens the artificial bioluminescent bacterium was used - geneticallymodified Escherichia coli K12 that carries Photorhabdus genes for bioluminescence. Both of these G- bacteria can be used for antibacterial assays based on their bioluminescence ability. Bioluminescence reaction is mediated by bacterial enzyme luciferasewhich catalyses the oxidation of long-chain aldehyde (substrate) and reduces flavin mononucleotide with emission of light. This emission can be immediately measured by the luminometer.

Druh

O - Ostatní výsledky

CEP obor

EC - Imunologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GP206%2F09%2FP470" target="_blank" >GP206/09/P470: Vliv inhibitorů biosyntézy eikosanoidů na imunitu zavíječe voskového Galleria mellonella</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů