Fe/Mn superoxide dismutase in Paracoccus denitrificans is induced by azide and expressed independently on FNR-type regulators

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00049905" target="_blank" >RIV/00216224:14310/11:00049905 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s12223-011-0007-3" target="_blank" >http://dx.doi.org/10.1007/s12223-011-0007-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-011-0007-3" target="_blank" >10.1007/s12223-011-0007-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fe/Mn superoxide dismutase in Paracoccus denitrificans is induced by azide and expressed independently on FNR-type regulators

Popis výsledku v původním jazyce

In the facultatively anaerobic bacterium Paracoccus denitrificans, the synthesis of many proteins involved in energy metabolism is regulated by the FNR-type transcription factors (FnrP, NNR and NarR). One of them, NarR, senses nitrate or nitrite concentration and promotes expression of the membrane-bound nitrate reductase genes. It can also be activated by azide as a non-metabolizable analogue of nitrate. In this work we show that one of the most prominent protein species inducible by azide is a Fe/Mn-family superoxide dismutase (SOD). Azide induces SOD at protein, mRNA transcript and enzyme activity levels in the aerobically growing cells. Since SOD expression remains unaffected in the fnrP, nnr and narR mutant strains, we postulate a mechanism independent of the known FNR-type regulators but involving a redox signal arising from the respiratory chain.

Název v anglickém jazyce

Fe/Mn superoxide dismutase in Paracoccus denitrificans is induced by azide and expressed independently on FNR-type regulators

Popis výsledku anglicky

In the facultatively anaerobic bacterium Paracoccus denitrificans, the synthesis of many proteins involved in energy metabolism is regulated by the FNR-type transcription factors (FnrP, NNR and NarR). One of them, NarR, senses nitrate or nitrite concentration and promotes expression of the membrane-bound nitrate reductase genes. It can also be activated by azide as a non-metabolizable analogue of nitrate. In this work we show that one of the most prominent protein species inducible by azide is a Fe/Mn-family superoxide dismutase (SOD). Azide induces SOD at protein, mRNA transcript and enzyme activity levels in the aerobically growing cells. Since SOD expression remains unaffected in the fnrP, nnr and narR mutant strains, we postulate a mechanism independent of the known FNR-type regulators but involving a redox signal arising from the respiratory chain.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Microbiologica

ISSN

0015-5632

e-ISSN

—

Svazek periodika

56

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

5

Strana od-do

13-17

Kód UT WoS článku

000290451500003

EID výsledku v databázi Scopus

—