Commonly used ROS-sensitive fluorescent probes are substrates of ABC transporters

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00050554" target="_blank" >RIV/00216224:14310/11:00050554 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Commonly used ROS-sensitive fluorescent probes are substrates of ABC transporters

Popis výsledku v původním jazyce

Intracellular ROS production significantly regulates the fate of various cell types through redox interaction with signal transduction pathways (reviewed in Hensley et al. 2000). Thus, the determination of the actual intracellular ROS level in cells is crucial for understanding the ROS role in the key processes governing differentiation, cellular death and carcinogenesis. Intracellular ROS are usually produced by non-phagocytic NADPH oxidases and mitochondria (Kowaltowski et al. 2009). Thus, mitochondria mass and membrane potential are often determined together with intracellular ROS production. A wide spectrum of fluorescent redox-sensitive probes for the determination of intracellular ROS production have been developed and employed in numerous studies (Soh 2006, Dickinson et al. 2009). Both the high sensitivity and the reproducibility of results of are directly connected with the preservation of the oxidized/reduced fluorescent probe inside the cell.

Název v anglickém jazyce

Commonly used ROS-sensitive fluorescent probes are substrates of ABC transporters

Popis výsledku anglicky

Intracellular ROS production significantly regulates the fate of various cell types through redox interaction with signal transduction pathways (reviewed in Hensley et al. 2000). Thus, the determination of the actual intracellular ROS level in cells is crucial for understanding the ROS role in the key processes governing differentiation, cellular death and carcinogenesis. Intracellular ROS are usually produced by non-phagocytic NADPH oxidases and mitochondria (Kowaltowski et al. 2009). Thus, mitochondria mass and membrane potential are often determined together with intracellular ROS production. A wide spectrum of fluorescent redox-sensitive probes for the determination of intracellular ROS production have been developed and employed in numerous studies (Soh 2006, Dickinson et al. 2009). Both the high sensitivity and the reproducibility of results of are directly connected with the preservation of the oxidized/reduced fluorescent probe inside the cell.

Druh

O - Ostatní výsledky

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA301%2F08%2F0717" target="_blank" >GA301/08/0717: Regulace diferenciace ES buněk prostřednictvím receptoru gp130</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů