ACID-BASE PROPERTIES OF CYTOSINE-CONTAINING OLIGONUCLEOTIDES

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00052407" target="_blank" >RIV/00216224:14310/11:00052407 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

ACID-BASE PROPERTIES OF CYTOSINE-CONTAINING OLIGONUCLEOTIDES

Popis výsledku v původním jazyce

The protonation equilibria1 of short-chain cytosine homo-oligodeoxyribonucleotides (ODNs) were studied by means of voltammetry and UV-Vis spectra. The work was aimed at ssDNA fragments with different length of the ODN chain. The shortest and longest ODNswere dC3 and dC18, respectively. In order to know the thermodynamic stability of ODNs, the effect of temperature and ionic strength was studied. The protonation constants were calculated by using a hard model (OPIUM or EQUISPEC) 2,3. It was found that the prolongation of the ODN chain supports the formation of more non-canonical bonds in the hemiprotonated (C+-C) pair (?i-motif?). While the exothermic protonation of nucleotides (CMP or dCMP) does not differ, the thermodynamic parameters change for ODNsas a consequence of the existence of an ?i-motif? confirmed also by elimination voltammetry with linear scan (EVLS)4. These facts are important for the knowledge of the secondary structure of ODNs containing C nucleobase.

Název v anglickém jazyce

ACID-BASE PROPERTIES OF CYTOSINE-CONTAINING OLIGONUCLEOTIDES

Popis výsledku anglicky

The protonation equilibria1 of short-chain cytosine homo-oligodeoxyribonucleotides (ODNs) were studied by means of voltammetry and UV-Vis spectra. The work was aimed at ssDNA fragments with different length of the ODN chain. The shortest and longest ODNswere dC3 and dC18, respectively. In order to know the thermodynamic stability of ODNs, the effect of temperature and ionic strength was studied. The protonation constants were calculated by using a hard model (OPIUM or EQUISPEC) 2,3. It was found that the prolongation of the ODN chain supports the formation of more non-canonical bonds in the hemiprotonated (C+-C) pair (?i-motif?). While the exothermic protonation of nucleotides (CMP or dCMP) does not differ, the thermodynamic parameters change for ODNsas a consequence of the existence of an ?i-motif? confirmed also by elimination voltammetry with linear scan (EVLS)4. These facts are important for the knowledge of the secondary structure of ODNs containing C nucleobase.

Druh

O - Ostatní výsledky

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

—

Projekt

<a href="/cs/project/LC06035" target="_blank" >LC06035: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů