Bisprimer?A Program for the Design of Primers for Bisulfite-Based Genomic Sequencing of Both Plant and Mammalian DNA Samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00059285" target="_blank" >RIV/00216224:14310/12:00059285 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/12:00383563

Výsledek na webu

<a href="http://jhered.oxfordjournals.org/content/early/2012/01/13/jhered.esr137.abstract" target="_blank" >http://jhered.oxfordjournals.org/content/early/2012/01/13/jhered.esr137.abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jhered/esr137" target="_blank" >10.1093/jhered/esr137</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Bisprimer?A Program for the Design of Primers for Bisulfite-Based Genomic Sequencing of Both Plant and Mammalian DNA Samples

Popis výsledku v původním jazyce

Plants and animals differ in the sequence context of the methylated sites in DNA. Plants exhibit cytosine methylation in CG, CHG, and CHH sites, whereas CG methylation is the only form present in mammals (with an exception of the early embryonic development). This fact must be taken into account in the design of primers for bisulfite-based genomic sequencing because CHG and CHH sites can remain unmodified. Surprisingly, no user-friendly primer design program is publicly available that could be used to design primers in plants and to simultaneously check the properties of primers such as the potential for primer-dimer formation. For studies concentrating on particular DNA loci, the correct design of primers is crucial. The program, called BisPrimer, includes 2 different subprograms for the primer design, the first one for mammals and the second one for angiosperm plants. Each subprogram is divided into 2 variants.

Název v anglickém jazyce

Bisprimer?A Program for the Design of Primers for Bisulfite-Based Genomic Sequencing of Both Plant and Mammalian DNA Samples

Popis výsledku anglicky

Plants and animals differ in the sequence context of the methylated sites in DNA. Plants exhibit cytosine methylation in CG, CHG, and CHH sites, whereas CG methylation is the only form present in mammals (with an exception of the early embryonic development). This fact must be taken into account in the design of primers for bisulfite-based genomic sequencing because CHG and CHH sites can remain unmodified. Surprisingly, no user-friendly primer design program is publicly available that could be used to design primers in plants and to simultaneously check the properties of primers such as the potential for primer-dimer formation. For studies concentrating on particular DNA loci, the correct design of primers is crucial. The program, called BisPrimer, includes 2 different subprograms for the primer design, the first one for mammals and the second one for angiosperm plants. Each subprogram is divided into 2 variants.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Heredity

ISSN

0022-1503

e-ISSN

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Svazek periodika

103

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

308-312

Kód UT WoS článku

000300723400017

EID výsledku v databázi Scopus

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