Caspase 3 chemiluminescence activity determination in apoptotic cells and design of caspase-3 sensor
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00072525" target="_blank" >RIV/00216224:14310/12:00072525 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Caspase 3 chemiluminescence activity determination in apoptotic cells and design of caspase-3 sensor
Popis výsledku v původním jazyce
Apoptosis, or programmed cell death, is an essential physiological process that plays a critical role in development of tumor or autoimmune disorders. In some instances knowledges of this pathway can be used for inhibit or prevent cancer development andother diseases. One of the earliest and most consistent observed features of apoptosis is the induction of a series of cytosolic proteases - caspases. Active caspases cleave numerous intracellular proteins and contribute to apoptotic cell death. Caspasesrecognize tetra-peptide sequences Asp-Glu-Val-Asp on their substrates and hydrolyze peptide bonds after aspartic acid residues. It is known from literature that in one apoptotic cell about 1.6x10(-19) mol of caspase can be activated. Various techniquesfor the determination of caspase 3 in free cells or tissue samples are commercially available. Caspase 3 activity is usually assayed by a chemiluminescence reaction. The best commercial methods reach the limit o detection higher than 1 pg
Název v anglickém jazyce
Caspase 3 chemiluminescence activity determination in apoptotic cells and design of caspase-3 sensor
Popis výsledku anglicky
Apoptosis, or programmed cell death, is an essential physiological process that plays a critical role in development of tumor or autoimmune disorders. In some instances knowledges of this pathway can be used for inhibit or prevent cancer development andother diseases. One of the earliest and most consistent observed features of apoptosis is the induction of a series of cytosolic proteases - caspases. Active caspases cleave numerous intracellular proteins and contribute to apoptotic cell death. Caspasesrecognize tetra-peptide sequences Asp-Glu-Val-Asp on their substrates and hydrolyze peptide bonds after aspartic acid residues. It is known from literature that in one apoptotic cell about 1.6x10(-19) mol of caspase can be activated. Various techniquesfor the determination of caspase 3 in free cells or tissue samples are commercially available. Caspase 3 activity is usually assayed by a chemiluminescence reaction. The best commercial methods reach the limit o detection higher than 1 pg
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů