Cellular ATP changes in Acidithiobacillus ferrooxidans cultures oxidizing ferrous iron and elemental sulfur

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00065972" target="_blank" >RIV/00216224:14310/13:00065972 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1080/01490451.2011.636790" target="_blank" >http://dx.doi.org/10.1080/01490451.2011.636790</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/01490451.2011.636790" target="_blank" >10.1080/01490451.2011.636790</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cellular ATP changes in Acidithiobacillus ferrooxidans cultures oxidizing ferrous iron and elemental sulfur

Popis výsledku v původním jazyce

Cellular ATP content of Acidithiobacillus ferrooxidans cultures was determined with a bioluminescence assay in relation to batch growth and oxidation of ferrous iron and elemental sulfur. Inhibitory effects of inorganic substrates and products on luciferase were eliminated. Extracellular ATP levels were negligible. The ATP content of sulfur-grown cells decreased anomalously due to a culture pH increase at the stationary phase. Although the rates of growth and sulfur oxidation reached the original levels, the ATP content of the culture remained constant because of gradual decrease in the cellular ATP. The maximum ATP levels in A. ferrooxidans grown with Fe(II) and S(0) were 1.16 and 0.33 amol per cell, respectively. The results defined conditions underwhich biomass growth could be monitored by the ATP assay to study biogeochemical activities of acidophilic iron- and sulfur-oxidizing bacteria.

Název v anglickém jazyce

Cellular ATP changes in Acidithiobacillus ferrooxidans cultures oxidizing ferrous iron and elemental sulfur

Popis výsledku anglicky

Cellular ATP content of Acidithiobacillus ferrooxidans cultures was determined with a bioluminescence assay in relation to batch growth and oxidation of ferrous iron and elemental sulfur. Inhibitory effects of inorganic substrates and products on luciferase were eliminated. Extracellular ATP levels were negligible. The ATP content of sulfur-grown cells decreased anomalously due to a culture pH increase at the stationary phase. Although the rates of growth and sulfur oxidation reached the original levels, the ATP content of the culture remained constant because of gradual decrease in the cellular ATP. The maximum ATP levels in A. ferrooxidans grown with Fe(II) and S(0) were 1.16 and 0.33 amol per cell, respectively. The results defined conditions underwhich biomass growth could be monitored by the ATP assay to study biogeochemical activities of acidophilic iron- and sulfur-oxidizing bacteria.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

DD - Geochemie

OECD FORD obor

—

Projekt

<a href="/cs/project/GA525%2F08%2F0697" target="_blank" >GA525/08/0697: Aktivita acidofilních bakterií oxidujících síru za aerobních a anaerobních podmínek</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Geomicrobiology Journal

ISSN

0149-0451

e-ISSN

—

Svazek periodika

30

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

1-7

Kód UT WoS článku

000311408900001

EID výsledku v databázi Scopus

—