REDOX STATE OF GLUTATHIONE DURING SHORT-TERM PHOTOINHIBITION AND RECOVERY IN FRUTICOSE AND FOLIOSE LICHEN SPECIES

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00069704" target="_blank" >RIV/00216224:14310/13:00069704 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

REDOX STATE OF GLUTATHIONE DURING SHORT-TERM PHOTOINHIBITION AND RECOVERY IN FRUTICOSE AND FOLIOSE LICHEN SPECIES

Popis výsledku v původním jazyce

Lichens possess several antioxidative enzymes and substrates that help them to overcome negative effects of photoinhibition of photosyntesis. Among them, glutathione, its redox state respectively, represents one of the mechanisms exploited to cope with strong high light stress. Effects of medium light stress on glutathione, however, have not yet been investigated in lichens. In our study, we addressed a question whether or not glutathione content alters in lichens exposed to medium light stress. Wet thalli of Usnea antarctica, U. aurantiaco-atra, and Pseudocyphellaria sp. were exposed to 800 micromol.m-2.s-1 PAR for 1 h at 5 oC. After the exposition, lichens were provided 6 h recovery at 10 micromol.m-2.s-1. Thalli segments were taken during the exposition each 10 min, and after 10, 30, 60 and 360 min during recovery. Frozen, lyophylizied, powdered and homogenized samples were diluted in HCl and disulphidic groups reduced by DTT. Thiol groups were labeled by mBBr.

Název v anglickém jazyce

REDOX STATE OF GLUTATHIONE DURING SHORT-TERM PHOTOINHIBITION AND RECOVERY IN FRUTICOSE AND FOLIOSE LICHEN SPECIES

Popis výsledku anglicky

Lichens possess several antioxidative enzymes and substrates that help them to overcome negative effects of photoinhibition of photosyntesis. Among them, glutathione, its redox state respectively, represents one of the mechanisms exploited to cope with strong high light stress. Effects of medium light stress on glutathione, however, have not yet been investigated in lichens. In our study, we addressed a question whether or not glutathione content alters in lichens exposed to medium light stress. Wet thalli of Usnea antarctica, U. aurantiaco-atra, and Pseudocyphellaria sp. were exposed to 800 micromol.m-2.s-1 PAR for 1 h at 5 oC. After the exposition, lichens were provided 6 h recovery at 10 micromol.m-2.s-1. Thalli segments were taken during the exposition each 10 min, and after 10, 30, 60 and 360 min during recovery. Frozen, lyophylizied, powdered and homogenized samples were diluted in HCl and disulphidic groups reduced by DTT. Thiol groups were labeled by mBBr.

Druh

O - Ostatní výsledky

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů