Interaction of oligonucleotides with benzo[c]phenanthridine alkaloid sanguilutine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00070284" target="_blank" >RIV/00216224:14310/13:00070284 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/article/10.2478%2Fs11696-013-0340-x" target="_blank" >http://link.springer.com/article/10.2478%2Fs11696-013-0340-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/s11696-013-0340-x" target="_blank" >10.2478/s11696-013-0340-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interaction of oligonucleotides with benzo[c]phenanthridine alkaloid sanguilutine

Popis výsledku v původním jazyce

Benzo[c]phenanthridine alkaloid sanguilutine (present in plant Sanguinaria canadensis) was extracted and separated from the plant material (dried roots). Interaction of the positively charged iminium form of alkaloid with double stranded DNA is reportedin this article. Significant changes in fluorescence properties in the presence or absence of various oligonucleotides have allowed us to study this system by means of luminescence spectroscopy. Results have shown that interaction with different double stranded oligonucleotides is not specific to A-T or G-C base pairs and also no preference of homogeneous or heterogeneous base composition of strands was found. Association constants (log K) were calculated to be in the range of 5.11 to 6.16. The responseof luminescence intensity at 610 nm to low concentrations of double stranded DNA was found to be linear and can be potentially used for fluorometric quantification of DNA. The limit of detection was estimated to be 120 ng.

Název v anglickém jazyce

Interaction of oligonucleotides with benzo[c]phenanthridine alkaloid sanguilutine

Popis výsledku anglicky

Benzo[c]phenanthridine alkaloid sanguilutine (present in plant Sanguinaria canadensis) was extracted and separated from the plant material (dried roots). Interaction of the positively charged iminium form of alkaloid with double stranded DNA is reportedin this article. Significant changes in fluorescence properties in the presence or absence of various oligonucleotides have allowed us to study this system by means of luminescence spectroscopy. Results have shown that interaction with different double stranded oligonucleotides is not specific to A-T or G-C base pairs and also no preference of homogeneous or heterogeneous base composition of strands was found. Association constants (log K) were calculated to be in the range of 5.11 to 6.16. The responseof luminescence intensity at 610 nm to low concentrations of double stranded DNA was found to be linear and can be potentially used for fluorometric quantification of DNA. The limit of detection was estimated to be 120 ng.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/LH12176" target="_blank" >LH12176: Benzofenanthridinové alkaloidy - studium účinků na celulární a molekulární úrovni</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemical Papers

ISSN

0366-6352

e-ISSN

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Svazek periodika

67

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

5

Strana od-do

568-572

Kód UT WoS článku

000314882900012

EID výsledku v databázi Scopus

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