Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00073256" target="_blank" >RIV/00216224:14310/14:00073256 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity

Popis výsledku v původním jazyce

Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis and Steinernema are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. These EPNs are symbiotically associated with bacteriaof the genera Photorhabdus or Xenorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest its tissues to provide nutrients for themselves and for expanding nematodes. We used EPNs infection to study immunity of Drosophila melanogaster and here we show for the first time that also honeybee larvae are suitable hosts for nematobacterial complex. We used the tripartite model (honeybee, nematodes, bacteria) to develop standard procedure for testing honeybees' immune response. We optimised the infection for EPN Heterorhabditis bacteriophora and Steinernema feltiae; both species cause typical coloration of cadavers (red or yellow, respectively), multiply in honeybee larvae and shows similar mortality wi

Název v anglickém jazyce

Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity

Popis výsledku anglicky

Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis and Steinernema are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. These EPNs are symbiotically associated with bacteriaof the genera Photorhabdus or Xenorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest its tissues to provide nutrients for themselves and for expanding nematodes. We used EPNs infection to study immunity of Drosophila melanogaster and here we show for the first time that also honeybee larvae are suitable hosts for nematobacterial complex. We used the tripartite model (honeybee, nematodes, bacteria) to develop standard procedure for testing honeybees' immune response. We optimised the infection for EPN Heterorhabditis bacteriophora and Steinernema feltiae; both species cause typical coloration of cadavers (red or yellow, respectively), multiply in honeybee larvae and shows similar mortality wi

Druh

O - Ostatní výsledky

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

<a href="/cs/project/QJ1210047" target="_blank" >QJ1210047: Vývoj nových prostředků pro podporu imunity včel, prevenci a léčbu včelích onemocnění</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů