EGFR signaling in the HGG-02 glioblastoma cell line with an unusual loss of EGFR gene copy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00074892" target="_blank" >RIV/00216224:14310/14:00074892 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00209805:_____/14:#0000498

Výsledek na webu

<a href="http://dx.doi.org/10.3892/or.2013.2864" target="_blank" >http://dx.doi.org/10.3892/or.2013.2864</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/or.2013.2864" target="_blank" >10.3892/or.2013.2864</a>

Jazyk výsledku

angličtina

Název v původním jazyce

EGFR signaling in the HGG-02 glioblastoma cell line with an unusual loss of EGFR gene copy

Popis výsledku v původním jazyce

Epidermal growth factor receptor (EGFR) gene amplification and the overexpression of EGFR are described as common features of glioblastoma multiforme (GBM). Nevertheless, we previously reported the loss of EGFR gene copy in a GBM specimen from a patientwith an unusually favorable course of the disease, and the HGG-02 cell line with this aberration was successfully derived from this tumor. Here, we present a detailed analysis of changes in gene expression and cell signaling in the HGG-02 cell line; theGM7 reference cell line with a standard EGFR gene copy number derived from a very aggressive GBM was used as a control. We confirmed the downregulation of EGFR expression and signaling in HGG-02 cells using different methods (RTK analysis, gene profilingand RT-PCR). Other changes that may have contributed to the non-aggressive phenotype of the primary tumor were identified, including the downregulated phosphorylation of the Axl and Trk receptors, as well as increased activity of JNK and

Název v anglickém jazyce

EGFR signaling in the HGG-02 glioblastoma cell line with an unusual loss of EGFR gene copy

Popis výsledku anglicky

Epidermal growth factor receptor (EGFR) gene amplification and the overexpression of EGFR are described as common features of glioblastoma multiforme (GBM). Nevertheless, we previously reported the loss of EGFR gene copy in a GBM specimen from a patientwith an unusually favorable course of the disease, and the HGG-02 cell line with this aberration was successfully derived from this tumor. Here, we present a detailed analysis of changes in gene expression and cell signaling in the HGG-02 cell line; theGM7 reference cell line with a standard EGFR gene copy number derived from a very aggressive GBM was used as a control. We confirmed the downregulation of EGFR expression and signaling in HGG-02 cells using different methods (RTK analysis, gene profilingand RT-PCR). Other changes that may have contributed to the non-aggressive phenotype of the primary tumor were identified, including the downregulated phosphorylation of the Axl and Trk receptors, as well as increased activity of JNK and

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Oncology Reports

ISSN

1021-335X

e-ISSN

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Svazek periodika

31

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GR - Řecká republika

Počet stran výsledku

8

Strana od-do

480-487

Kód UT WoS článku

000330788100065

EID výsledku v databázi Scopus

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