Study of the retention behavior of trinucleotides in HPLC

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Study of the retention behavior of trinucleotides in HPLC

Popis výsledku v původním jazyce

Trinucleotides are short DNA sequences of three nucleobases; in our study we used guanine, adenine and thymine. It was shown that a useful tool to study the effect of sequence on retention was achieved by reversed phase liquid chromatography in ion-interaction mode (II-RPLC). Retention mechanism in the II-RPLC is based on addition of the ion interaction agent (in a form of buffer ions) to the mobile phase, which can effectively compensate the charge on the phosphate groups of a sugar-phosphate backbone,thereby increasing the hydrophobicity and thus the retention in system with reversed phase. To study the retention behavior we have chosen a combination of experimental design (ED) and artificial neural networks (ANN). ED was used to target more variable parameters in parallel while monitoring the system response. This method allows to determine the size of the effect of the various factors involved in the retention and to possibly reveal their mutual correlation.

Název v anglickém jazyce

Study of the retention behavior of trinucleotides in HPLC

Popis výsledku anglicky

Trinucleotides are short DNA sequences of three nucleobases; in our study we used guanine, adenine and thymine. It was shown that a useful tool to study the effect of sequence on retention was achieved by reversed phase liquid chromatography in ion-interaction mode (II-RPLC). Retention mechanism in the II-RPLC is based on addition of the ion interaction agent (in a form of buffer ions) to the mobile phase, which can effectively compensate the charge on the phosphate groups of a sugar-phosphate backbone,thereby increasing the hydrophobicity and thus the retention in system with reversed phase. To study the retention behavior we have chosen a combination of experimental design (ED) and artificial neural networks (ANN). ED was used to target more variable parameters in parallel while monitoring the system response. This method allows to determine the size of the effect of the various factors involved in the retention and to possibly reveal their mutual correlation.

Druh

D - Stať ve sborníku

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Universitatis Palackianae Olomucensis Chemica 51S

ISBN

9788024439501

ISSN

0232-0061

e-ISSN

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Počet stran výsledku

2

Strana od-do

161-162

Název nakladatele

Palacký University, Olomouc

Místo vydání

Olomouc

Místo konání akce

Olomouc

Datum konání akce

10. 2. 2014

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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