Simultaneous measurements of PSII activity and thallus temperature during linear cooling. A method to assess interspecific differences in photosynthetic processes in lichens from polar regions.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00076590" target="_blank" >RIV/00216224:14310/14:00076590 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Simultaneous measurements of PSII activity and thallus temperature during linear cooling. A method to assess interspecific differences in photosynthetic processes in lichens from polar regions.

Popis výsledku v původním jazyce

A comparison of cryoresistance of isolated lichen photobiont and polar lichens is presented. Several species were chosen for this experiment: (1) algal cultures of Trebouxia glomerata, Trebouxia sp. (2) thalli of Usnea antarctica, Usnea aurantiaco-atra,Usnea longissima, Umbilicaria decussata, Umbilicaria cylindrica. All the samples were exposed to temperatures linearly decreasing from +20 °C to -40 °C under controlled conditions. During the experiment, sample temperature and two parameters of chlorophyll fluorescence ? (1) potential (FV/FM) and (2) effective quantum yield of photochemical processes in photosystem II (YieldPSII) ? were measured simultaneously. Cooling was provided by a KRYO 560-16 (PLANER, United Kingdom) device. For majority of samples, two cooling rates were used: 6 °C min-1 (fast) and 2 °C min-1 (slow) were used. The chlorophyll fluorescence was measured by a PC-linked PAM 2000 fluorometer (H. Walz, Germany). Measurements of YieldPSII were made every 30 s.

Název v anglickém jazyce

Simultaneous measurements of PSII activity and thallus temperature during linear cooling. A method to assess interspecific differences in photosynthetic processes in lichens from polar regions.

Popis výsledku anglicky

A comparison of cryoresistance of isolated lichen photobiont and polar lichens is presented. Several species were chosen for this experiment: (1) algal cultures of Trebouxia glomerata, Trebouxia sp. (2) thalli of Usnea antarctica, Usnea aurantiaco-atra,Usnea longissima, Umbilicaria decussata, Umbilicaria cylindrica. All the samples were exposed to temperatures linearly decreasing from +20 °C to -40 °C under controlled conditions. During the experiment, sample temperature and two parameters of chlorophyll fluorescence ? (1) potential (FV/FM) and (2) effective quantum yield of photochemical processes in photosystem II (YieldPSII) ? were measured simultaneously. Cooling was provided by a KRYO 560-16 (PLANER, United Kingdom) device. For majority of samples, two cooling rates were used: 6 °C min-1 (fast) and 2 °C min-1 (slow) were used. The chlorophyll fluorescence was measured by a PC-linked PAM 2000 fluorometer (H. Walz, Germany). Measurements of YieldPSII were made every 30 s.

Druh

O - Ostatní výsledky

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

<a href="/cs/project/LM2010009" target="_blank" >LM2010009: Projekt CzechPolar - České polární stanice: Stavba a operační náklady</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů