MALDI-TOF fingerprinting of the whole mammalian cell-lines

Kód výsledku v IS VaVaI

RIV/00216224:14310/14:00078221 - isvavai.cz

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

MALDI-TOF fingerprinting of the whole mammalian cell-lines

Popis výsledku v původním jazyce

Robust cell-lines identification requires reliable databases of cell-lines fingerprints. Mass spectrometric fingerprinting of mammalian cell-lines is a promising way to achieve the goal of fast, cheap, simple and reliable cell-lines authentication. However, general and standardized method for mass spectrometric fingerprinting of the whole mammalian cell-lines is not available to date. We developed, optimized and validated MALDI-TOF mass spectrometric method for reliable, specific and reproducible whole-cell fingerprinting of mammalian cell-lines. Quality and robustness of mass spectrometric fingerprints is demonstrated by statistical analysis of experimental data. Developed method for the whole mammalian cell-lines fingerprinting, in contrast to actualprocedures, is inexpensive, fast and it opens the door towards routine cell authentication.

Název v anglickém jazyce

MALDI-TOF fingerprinting of the whole mammalian cell-lines

Popis výsledku anglicky

Robust cell-lines identification requires reliable databases of cell-lines fingerprints. Mass spectrometric fingerprinting of mammalian cell-lines is a promising way to achieve the goal of fast, cheap, simple and reliable cell-lines authentication. However, general and standardized method for mass spectrometric fingerprinting of the whole mammalian cell-lines is not available to date. We developed, optimized and validated MALDI-TOF mass spectrometric method for reliable, specific and reproducible whole-cell fingerprinting of mammalian cell-lines. Quality and robustness of mass spectrometric fingerprints is demonstrated by statistical analysis of experimental data. Developed method for the whole mammalian cell-lines fingerprinting, in contrast to actualprocedures, is inexpensive, fast and it opens the door towards routine cell authentication.

Druh

O - Ostatní výsledky

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

ED2.1.00/03.0086: Regionální VaV centrum pro nízkonákladové plazmové a nanotechnologické povrchové úpravy

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů