Role of CD36 and GPR120 in fatty acid-mediated Ca2+Signaling in human and mouse taste bud cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00086887" target="_blank" >RIV/00216224:14310/14:00086887 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1093/chemse/bju073" target="_blank" >http://dx.doi.org/10.1093/chemse/bju073</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/chemse/bju073" target="_blank" >10.1093/chemse/bju073</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Role of CD36 and GPR120 in fatty acid-mediated Ca2+Signaling in human and mouse taste bud cells

Popis výsledku v původním jazyce

Oral perception of dietary fat was until recently thought to involve mainly texture and olfactory cues; however, accumulating evidence strongly suggests existence of a taste modality, devoted to the detection of long-chain fatty acids (LCFA). Mice can recognize dietary fat and FA solutions in the oral cavity in the absence of olfactory or textural cues. Hence, it is important to increase our understanding of gustatory detection of dietary fat and its contribution to fat preference. We studied the rolesof the fat taste receptors CD36 and GPR120 and their interactions via Ca2+ signaling in fungiform taste bud cells (TBC). We measured Ca2+ signaling in human TBC, transfected with small interfering RNAs (siRNAs) against mRNAs encoding CD36 and GPR120 (orcontrol siRNAs). We also studied Ca2+signaling in TBC from CD36?/? mice and from wild-type lean and obese mice. Additional studies were conducted with mouse enteroendocrine cell line STC-1 that express GPR120 and stably transfected with h

Název v anglickém jazyce

Role of CD36 and GPR120 in fatty acid-mediated Ca2+Signaling in human and mouse taste bud cells

Popis výsledku anglicky

Oral perception of dietary fat was until recently thought to involve mainly texture and olfactory cues; however, accumulating evidence strongly suggests existence of a taste modality, devoted to the detection of long-chain fatty acids (LCFA). Mice can recognize dietary fat and FA solutions in the oral cavity in the absence of olfactory or textural cues. Hence, it is important to increase our understanding of gustatory detection of dietary fat and its contribution to fat preference. We studied the rolesof the fat taste receptors CD36 and GPR120 and their interactions via Ca2+ signaling in fungiform taste bud cells (TBC). We measured Ca2+ signaling in human TBC, transfected with small interfering RNAs (siRNAs) against mRNAs encoding CD36 and GPR120 (orcontrol siRNAs). We also studied Ca2+signaling in TBC from CD36?/? mice and from wild-type lean and obese mice. Additional studies were conducted with mouse enteroendocrine cell line STC-1 that express GPR120 and stably transfected with h

Druh

D - Stať ve sborníku

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Chemical Senses

ISBN

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ISSN

0379-864X

e-ISSN

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Počet stran výsledku

2

Strana od-do

277-278

Název nakladatele

Oxford University Press

Místo vydání

Anglie

Místo konání akce

Francie

Datum konání akce

10. 9. 2014

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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