Evaluation of the MALDI-TOF MS profiling for identification of newly described Aeromonas spp.
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00080481" target="_blank" >RIV/00216224:14310/15:00080481 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1007/s12223-014-0369-4" target="_blank" >http://dx.doi.org/10.1007/s12223-014-0369-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12223-014-0369-4" target="_blank" >10.1007/s12223-014-0369-4</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Evaluation of the MALDI-TOF MS profiling for identification of newly described Aeromonas spp.
Popis výsledku v původním jazyce
The genus Aeromonas comprises primarily aquatic bacteria and also serious human and animal pathogens with the occurrence in clinical material, drinking water, and food. Aeromonads are typical for their complex taxonomy and nomenclature and for limited possibilities of identification to the species level. According to studies describing the use of MALDI-TOF MS in diagnostics of aeromonads, this modern chemotaxonomical approach reveals quite high percentage of correctly identified isolates. We analyzed 64Aeromonas reference strains from the set of 27 species. After extending the range of analyzed Aeromonas species by newly described ones, we proved that MALDI-TOF MS procedure accompanied by Biotyper tool is not a reliable diagnostic technique for aeromonads. We obtained quite high percentage of false-positive, incorrect, and uncertain results. The identification of newly described species is accompanied with misidentifications that were observed also in the case of pathogenic aeromonads
Název v anglickém jazyce
Evaluation of the MALDI-TOF MS profiling for identification of newly described Aeromonas spp.
Popis výsledku anglicky
The genus Aeromonas comprises primarily aquatic bacteria and also serious human and animal pathogens with the occurrence in clinical material, drinking water, and food. Aeromonads are typical for their complex taxonomy and nomenclature and for limited possibilities of identification to the species level. According to studies describing the use of MALDI-TOF MS in diagnostics of aeromonads, this modern chemotaxonomical approach reveals quite high percentage of correctly identified isolates. We analyzed 64Aeromonas reference strains from the set of 27 species. After extending the range of analyzed Aeromonas species by newly described ones, we proved that MALDI-TOF MS procedure accompanied by Biotyper tool is not a reliable diagnostic technique for aeromonads. We obtained quite high percentage of false-positive, incorrect, and uncertain results. The identification of newly described species is accompanied with misidentifications that were observed also in the case of pathogenic aeromonads
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/QI101A094" target="_blank" >QI101A094: Metody tlumení produkčních chorob skotu - BVD-MD a paratuberkulóza</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Folia Microbiologica
ISSN
0015-5632
e-ISSN
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Svazek periodika
60
Číslo periodika v rámci svazku
5
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
9
Strana od-do
375-383
Kód UT WoS článku
000359398900002
EID výsledku v databázi Scopus
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