Determination of pyruvate and lactate as potential biomarkers of embryo viability in assisted reproduction by capillary electrophoresis with contactless conductivity detection
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00080624" target="_blank" >RIV/00216224:14310/15:00080624 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1002/elps.201400487" target="_blank" >http://dx.doi.org/10.1002/elps.201400487</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201400487" target="_blank" >10.1002/elps.201400487</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Determination of pyruvate and lactate as potential biomarkers of embryo viability in assisted reproduction by capillary electrophoresis with contactless conductivity detection
Popis výsledku v původním jazyce
Importance of human assisted reproduction is rising as a response for rising number of couples suffering from infertility. The key steps in human assisted reproduction are in vitro fertilization of an oocyte, cultivation of embryo and selection of an embryo with the highest developmental potential for implantation. Assessment of developmental potential of embryo is lacking of precision thus novel techniques are sought. Capillary electrophoresis with contactless conductivity detection was applied for analysis of pyruvate and lactate as potential biomarkers of developmental potential of human embryos. Optimizations of separation conditions and treatment of a sample are described and discussed. Developed method was successfully applied for analysis of several samples of spent culture media collected after cultivation of embryos.
Název v anglickém jazyce
Determination of pyruvate and lactate as potential biomarkers of embryo viability in assisted reproduction by capillary electrophoresis with contactless conductivity detection
Popis výsledku anglicky
Importance of human assisted reproduction is rising as a response for rising number of couples suffering from infertility. The key steps in human assisted reproduction are in vitro fertilization of an oocyte, cultivation of embryo and selection of an embryo with the highest developmental potential for implantation. Assessment of developmental potential of embryo is lacking of precision thus novel techniques are sought. Capillary electrophoresis with contactless conductivity detection was applied for analysis of pyruvate and lactate as potential biomarkers of developmental potential of human embryos. Optimizations of separation conditions and treatment of a sample are described and discussed. Developed method was successfully applied for analysis of several samples of spent culture media collected after cultivation of embryos.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Electrophoresis
ISSN
0173-0835
e-ISSN
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Svazek periodika
36
Číslo periodika v rámci svazku
11-12
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
7
Strana od-do
1244-1250
Kód UT WoS článku
000356004200003
EID výsledku v databázi Scopus
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