ESEM observations of basic groups of parasites with note on the interesting mermithid nematode from Caerostris sumatrana Strand, 1915

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00080684" target="_blank" >RIV/00216224:14310/15:00080684 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

ESEM observations of basic groups of parasites with note on the interesting mermithid nematode from Caerostris sumatrana Strand, 1915

Popis výsledku v původním jazyce

This study introduces modified method of ESEM for observing representatives of the selected groups of helminth parasites from fish and spider. The most suitable treatment methods and optimal environmental condition for observing selected parasites were found. Already fixed samples (70% ethanol) were washed and rinsed with distilled water. Observations were made on a non-commercial ESEM AQUASEM II [6]. Samples were taken to the Peltier cooled specimen holder with silicon surface, into a drop of water. Observation conditions were: beam accelerating voltage 20 kV, probe current 110 pA, sample temperature 2 °C, water vapour pressure 650-700 Pa. Here, we demonstrated this method on the fish parasitic helminths and also on the subadult of mermithid parasite(Nematoda: Mermithidae). It was found parasitizing Caerostris sumatrana (Araneae: Araneidae), orbweb species of spider living in shrubs and trees of lowland dipterocarp forests.

Název v anglickém jazyce

ESEM observations of basic groups of parasites with note on the interesting mermithid nematode from Caerostris sumatrana Strand, 1915

Popis výsledku anglicky

This study introduces modified method of ESEM for observing representatives of the selected groups of helminth parasites from fish and spider. The most suitable treatment methods and optimal environmental condition for observing selected parasites were found. Already fixed samples (70% ethanol) were washed and rinsed with distilled water. Observations were made on a non-commercial ESEM AQUASEM II [6]. Samples were taken to the Peltier cooled specimen holder with silicon surface, into a drop of water. Observation conditions were: beam accelerating voltage 20 kV, probe current 110 pA, sample temperature 2 °C, water vapour pressure 650-700 Pa. Here, we demonstrated this method on the fish parasitic helminths and also on the subadult of mermithid parasite(Nematoda: Mermithidae). It was found parasitizing Caerostris sumatrana (Araneae: Araneidae), orbweb species of spider living in shrubs and trees of lowland dipterocarp forests.

Druh

O - Ostatní výsledky

CEP obor

EG - Zoologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GBP505%2F12%2FG112" target="_blank" >GBP505/12/G112: ECIP - Evropské centrum ichtyoparazitologie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů