Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00080746" target="_blank" >RIV/00216224:14310/15:00080746 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/65269705:_____/15:00063689 RIV/68081707:_____/15:00472803 RIV/61388971:_____/15:00472803

Výsledek na webu

<a href="http://dx.doi.org/10.3402/jev.v4.25530" target="_blank" >http://dx.doi.org/10.3402/jev.v4.25530</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3402/jev.v4.25530" target="_blank" >10.3402/jev.v4.25530</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer

Popis výsledku v původním jazyce

Flow cytometry is a powerful method, which is widely used for high-throughput quantitative and qualitative analysis of cells. However, its straightforward applicability for extracellular vesicles (EVs) and mainly exosomes is hampered by several challenges, reflecting mostly the small size of these vesicles (exosomes: similar to 80-200 nm, microvesicles: similar to 200-1,000 nm), their polydispersity, and low refractive index. The current best and most widely used protocol for beads-free flow cytometry of exosomes uses ultracentrifugation (UC) coupled with floatation in sucrose gradient for their isolation, labeling with lipophilic dye PKH67 and antibodies, and an optimized version of commercial high-end cytometer for analysis. However, this approach requires an experienced flow cytometer operator capable of manual hardware adjustments and calibration of the cytometer.

Název v anglickém jazyce

Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer

Popis výsledku anglicky

Flow cytometry is a powerful method, which is widely used for high-throughput quantitative and qualitative analysis of cells. However, its straightforward applicability for extracellular vesicles (EVs) and mainly exosomes is hampered by several challenges, reflecting mostly the small size of these vesicles (exosomes: similar to 80-200 nm, microvesicles: similar to 200-1,000 nm), their polydispersity, and low refractive index. The current best and most widely used protocol for beads-free flow cytometry of exosomes uses ultracentrifugation (UC) coupled with floatation in sucrose gradient for their isolation, labeling with lipophilic dye PKH67 and antibodies, and an optimized version of commercial high-end cytometer for analysis. However, this approach requires an experienced flow cytometer operator capable of manual hardware adjustments and calibration of the cytometer.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Extracellular Vesicles

ISSN

2001-3078

e-ISSN

—

Svazek periodika

4

Číslo periodika v rámci svazku

25530

Stát vydavatele periodika

SE - Švédské království

Počet stran výsledku

15

Strana od-do

1-15

Kód UT WoS článku

000365076000001

EID výsledku v databázi Scopus

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