Transcriptomic analyses of a monogenean: Next-Generation Sequencing techniques in hands of parasitologists.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00080923" target="_blank" >RIV/00216224:14310/15:00080923 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Transcriptomic analyses of a monogenean: Next-Generation Sequencing techniques in hands of parasitologists.

Popis výsledku v původním jazyce

Next-generation sequencing methods currently represent a robust tool enabling significant enrichment of our knowledge of biological systems at a level which has not been possible ever before. Unfortunately, the transcriptomic/genomic data on members of Monogenea are still extremely limited. Therefore we adopted the NGS techniques to generate a first high quality transcriptome of a representative of blood-feeding monogeneans - Eudiplozoon nipponicum (Diplozoidae: Heteronchoinea), the ectoparasite from the gills of common carp Cyprinus carpio. Using 454 sequencing technology (GS FLX System, Roche), a total of 324 941 reads were generated (average length 423 bp) and assembled into 6910 contigs. The second transcriptome analysis was performed by the MiSeqSystem (Illumina). A total of 74 823 255 input read pairs, a total of 142 813 all transcripts (median contig length 367 bp) and a total of 119 136 of genes (median contig length 334 bp) were obtained.

Název v anglickém jazyce

Transcriptomic analyses of a monogenean: Next-Generation Sequencing techniques in hands of parasitologists.

Popis výsledku anglicky

Next-generation sequencing methods currently represent a robust tool enabling significant enrichment of our knowledge of biological systems at a level which has not been possible ever before. Unfortunately, the transcriptomic/genomic data on members of Monogenea are still extremely limited. Therefore we adopted the NGS techniques to generate a first high quality transcriptome of a representative of blood-feeding monogeneans - Eudiplozoon nipponicum (Diplozoidae: Heteronchoinea), the ectoparasite from the gills of common carp Cyprinus carpio. Using 454 sequencing technology (GS FLX System, Roche), a total of 324 941 reads were generated (average length 423 bp) and assembled into 6910 contigs. The second transcriptome analysis was performed by the MiSeqSystem (Illumina). A total of 74 823 255 input read pairs, a total of 142 813 all transcripts (median contig length 367 bp) and a total of 119 136 of genes (median contig length 334 bp) were obtained.

Druh

O - Ostatní výsledky

CEP obor

EG - Zoologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů