The use of flower-like gold nanoparticles in time-of-flight mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00082770" target="_blank" >RIV/00216224:14310/15:00082770 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The use of flower-like gold nanoparticles in time-of-flight mass spectrometry

Popis výsledku v původním jazyce

Various types of gold nanoparticles (AuNPs) represent promising tools in many biomedical applications.[1-2] Here we report the first use of flower-like (FL) AuNPs as a mediator enhancing ionization in mass spectrometry (MS) of biomolecules. The main objective was to determine the effect of FL AuNPs on the ionization of biomolecules by surface assisted and matrix assisted laser desorption ionization (SALDI and MALDI) MS with time-of-flight analyzer. FL AuNPs alone or combined with cyano-4-hydroxycinnamicacid (CHCA) for MS measurement of peptides (500-3 500 m/z) were used. The comparison with classical CHCA matrix shows that FL AuNPs increase the ionization (~ 7.5 x) of peptides both alone and in combination with CHCA matrix. For ionization of higher mass peptides/proteins and those from mouse embryonic fibroblast (MEF) cells, FL AuNPs combined with CHCA and sinapinic acid (SA) were used.

Název v anglickém jazyce

The use of flower-like gold nanoparticles in time-of-flight mass spectrometry

Popis výsledku anglicky

Various types of gold nanoparticles (AuNPs) represent promising tools in many biomedical applications.[1-2] Here we report the first use of flower-like (FL) AuNPs as a mediator enhancing ionization in mass spectrometry (MS) of biomolecules. The main objective was to determine the effect of FL AuNPs on the ionization of biomolecules by surface assisted and matrix assisted laser desorption ionization (SALDI and MALDI) MS with time-of-flight analyzer. FL AuNPs alone or combined with cyano-4-hydroxycinnamicacid (CHCA) for MS measurement of peptides (500-3 500 m/z) were used. The comparison with classical CHCA matrix shows that FL AuNPs increase the ionization (~ 7.5 x) of peptides both alone and in combination with CHCA matrix. For ionization of higher mass peptides/proteins and those from mouse embryonic fibroblast (MEF) cells, FL AuNPs combined with CHCA and sinapinic acid (SA) were used.

Druh

O - Ostatní výsledky

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/EE2.3.20.0185" target="_blank" >EE2.3.20.0185: Centrum analýz a modelování tkání a orgánů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů