Polymer-immobilized ready-to-use recombinant yeast assays for the detection of endocrine disruptive compounds

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00086633" target="_blank" >RIV/00216224:14310/15:00086633 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0045653515001873" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0045653515001873</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chemosphere.2015.02.063" target="_blank" >10.1016/j.chemosphere.2015.02.063</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Polymer-immobilized ready-to-use recombinant yeast assays for the detection of endocrine disruptive compounds

Popis výsledku v původním jazyce

Recombinant yeast assays (RYAs) constitute a suitable tool for the environmental monitoring of compounds with endocrine disrupting activities, notably estrogenicity and androgenicity. Conventional procedures require yeast reconstitution from frozen stock, which usually takes several days and demands additional equipment With the aim of applying such assays to field studies and making them more accessible to less well-equipped laboratories, we have optimized RYA by the immobilization of Saccharomyces cerevisiae cells in three different polymer matrices gelatin, Bacto agar, and Yeast Extract Peptone Dextrose agar to obtain a ready-to-use version for the fast assessment of estrogenic and androgenic potencies of compounds and environmental samples. Among the three matrices, gelatin showed the best results for both testosterone (androgen receptor yeast strain; AR-RYA) and 17 beta-estradiol (estrogen receptor yeast strain; ER-RYA).

Název v anglickém jazyce

Polymer-immobilized ready-to-use recombinant yeast assays for the detection of endocrine disruptive compounds

Popis výsledku anglicky

Recombinant yeast assays (RYAs) constitute a suitable tool for the environmental monitoring of compounds with endocrine disrupting activities, notably estrogenicity and androgenicity. Conventional procedures require yeast reconstitution from frozen stock, which usually takes several days and demands additional equipment With the aim of applying such assays to field studies and making them more accessible to less well-equipped laboratories, we have optimized RYA by the immobilization of Saccharomyces cerevisiae cells in three different polymer matrices gelatin, Bacto agar, and Yeast Extract Peptone Dextrose agar to obtain a ready-to-use version for the fast assessment of estrogenic and androgenic potencies of compounds and environmental samples. Among the three matrices, gelatin showed the best results for both testosterone (androgen receptor yeast strain; AR-RYA) and 17 beta-estradiol (estrogen receptor yeast strain; ER-RYA).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

DN - Vliv životního prostředí na zdraví

OECD FORD obor

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Projekt

<a href="/cs/project/LO1214" target="_blank" >LO1214: Centrum pro výzkum toxických látek v prostředí</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemosphere

ISSN

0045-6535

e-ISSN

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Svazek periodika

132

Číslo periodika v rámci svazku

AUGUST

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

56-62

Kód UT WoS článku

000355882900008

EID výsledku v databázi Scopus

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