PHYLOGENETIC ANALYSIS OF CULTIVATION-RESISTANT TERRESTRIAL CYANOBACTERIA WITH MASSIVE SHEATHS (STIGONEMA SPP. AND PETALONEMA ALATUM, NOSTOCALES, CYANOBACTERIA) USING SINGLE-CELL AND FILAMENT SEQUENCING OF ENVIRONMENTAL SAMPLES
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00087123" target="_blank" >RIV/00216224:14310/15:00087123 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/67985939:_____/15:00447943 RIV/60076658:12310/15:43888731
Výsledek na webu
<a href="http://dx.doi.org/10.1111/jpy.12273" target="_blank" >http://dx.doi.org/10.1111/jpy.12273</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jpy.12273" target="_blank" >10.1111/jpy.12273</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
PHYLOGENETIC ANALYSIS OF CULTIVATION-RESISTANT TERRESTRIAL CYANOBACTERIA WITH MASSIVE SHEATHS (STIGONEMA SPP. AND PETALONEMA ALATUM, NOSTOCALES, CYANOBACTERIA) USING SINGLE-CELL AND FILAMENT SEQUENCING OF ENVIRONMENTAL SAMPLES
Popis výsledku v původním jazyce
Molecular assessment of a large portion of traditional cyanobacterial taxa has been hindered by the failure to isolate and grow them in culture. In this study, we developed an optimized protocol for single cell/filament isolation and 16S rRNA gene sequencing of terrestrial cyanobacteria with large mucilaginous sheaths, and applied it to determine the phylogenetic position of typical members of the genera Petalonema and Stigonema. A methodology based on a glass-capillary isolation technique and a semi-nested PCR protocol enabled reliable sequencing of the 16S rRNA gene from all samples analysed. Ten samples covering 7 species of Stigonema from Europe, North and Central America, and Hawaii, and the type species of Petalonema from Slovakia were sequenced.Contrary to some previous studies, which proposed a relation to heteropolar nostocalean cyanobacteria, Petalonema appeared to belong to the family Scytonemataceae.
Název v anglickém jazyce
PHYLOGENETIC ANALYSIS OF CULTIVATION-RESISTANT TERRESTRIAL CYANOBACTERIA WITH MASSIVE SHEATHS (STIGONEMA SPP. AND PETALONEMA ALATUM, NOSTOCALES, CYANOBACTERIA) USING SINGLE-CELL AND FILAMENT SEQUENCING OF ENVIRONMENTAL SAMPLES
Popis výsledku anglicky
Molecular assessment of a large portion of traditional cyanobacterial taxa has been hindered by the failure to isolate and grow them in culture. In this study, we developed an optimized protocol for single cell/filament isolation and 16S rRNA gene sequencing of terrestrial cyanobacteria with large mucilaginous sheaths, and applied it to determine the phylogenetic position of typical members of the genera Petalonema and Stigonema. A methodology based on a glass-capillary isolation technique and a semi-nested PCR protocol enabled reliable sequencing of the 16S rRNA gene from all samples analysed. Ten samples covering 7 species of Stigonema from Europe, North and Central America, and Hawaii, and the type species of Petalonema from Slovakia were sequenced.Contrary to some previous studies, which proposed a relation to heteropolar nostocalean cyanobacteria, Petalonema appeared to belong to the family Scytonemataceae.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EF - Botanika
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Phycology
ISSN
0022-3646
e-ISSN
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Svazek periodika
51
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
10
Strana od-do
288-297
Kód UT WoS článku
000352617500009
EID výsledku v databázi Scopus
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