Simplified direct Kjeldahl method suitable as a primary reference procedure for the determination of total protein in reference materials used in clinical chemistry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00108650" target="_blank" >RIV/00216224:14310/15:00108650 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.cskb.cz/res/file/KBM-pdf/2015/2015-4/KBM-4-2015-Chromy-188.pdf" target="_blank" >http://www.cskb.cz/res/file/KBM-pdf/2015/2015-4/KBM-4-2015-Chromy-188.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Simplified direct Kjeldahl method suitable as a primary reference procedure for the determination of total protein in reference materials used in clinical chemistry

Popis výsledku v původním jazyce

Chromý et al. found in 2009, that albumin-calibrated analysis of serum total protein with biuret reagent according to a candidate reference method developed by Doumas working group in 1981, provide results with unacceptable positive bias, caused namely by endogeneous serum bilirubin and lipids. They also found, that a simplest way to rectify this defect, is the use of serum and/or plasma-based protein standards, commutable with patients samples, which are certified by the Kjeldahl method. In our foregoing two articles, (Chromý et al., 2015, and Vinklárková et al., 2015), we reviewed Kjeldahl methods adopted by laboratory medicine, selected a direct analysis on washed protein precipitates with trichloroacetic acid and verified its preliminary analytical performance parameters. In this article, we describe a simplified Kjeldahl analysis with modified digestion flask, now directly connectable to accordingly adapted Parnas and Wagner distillation unit, and present its re-validation. Simplified Kjeldahl method is suggested as a standard operating procedure for total protein in reference materials used in clinical chemistry

Název v anglickém jazyce

Simplified direct Kjeldahl method suitable as a primary reference procedure for the determination of total protein in reference materials used in clinical chemistry

Popis výsledku anglicky

Chromý et al. found in 2009, that albumin-calibrated analysis of serum total protein with biuret reagent according to a candidate reference method developed by Doumas working group in 1981, provide results with unacceptable positive bias, caused namely by endogeneous serum bilirubin and lipids. They also found, that a simplest way to rectify this defect, is the use of serum and/or plasma-based protein standards, commutable with patients samples, which are certified by the Kjeldahl method. In our foregoing two articles, (Chromý et al., 2015, and Vinklárková et al., 2015), we reviewed Kjeldahl methods adopted by laboratory medicine, selected a direct analysis on washed protein precipitates with trichloroacetic acid and verified its preliminary analytical performance parameters. In this article, we describe a simplified Kjeldahl analysis with modified digestion flask, now directly connectable to accordingly adapted Parnas and Wagner distillation unit, and present its re-validation. Simplified Kjeldahl method is suggested as a standard operating procedure for total protein in reference materials used in clinical chemistry

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Klinická biochemie a metabolismus

ISSN

1210-7921

e-ISSN

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Svazek periodika

23

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

5

Strana od-do

188-192

Kód UT WoS článku

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EID výsledku v databázi Scopus

2-s2.0-84951044203