RADIOMETRIC DETERMINATION OF THYROTOXIC EFFECTS OF SOME XENOBIOTICS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00116081" target="_blank" >RIV/00216224:14310/15:00116081 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/15:00472832

Výsledek na webu

<a href="https://www.rad-conference.org/pdf/Proceedings%20RAD%202015.pdf" target="_blank" >https://www.rad-conference.org/pdf/Proceedings%20RAD%202015.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

RADIOMETRIC DETERMINATION OF THYROTOXIC EFFECTS OF SOME XENOBIOTICS

Popis výsledku v původním jazyce

Recently, we have shown in the isolated rat thyroids marked effects of excessive exogenous bromide and perchlorate ions on the activity of thyroid peroxidase (TPO), the key enzyme in thyroid hormones (TH) metabolism. Here, we studied in more details, with the aid of several radioanalytical methods, effects of an enhanced bromide and/or perchlorate intake on various aspects of iodine metabolism and, consequently, on TH metabolism in the rat. Goitrogenic and thyrotoxic effects of excessive bromide and perchlorate ions were followed in adult male rats, maintained on diets with various iodine content, ensuring either sufficient iodine supply or mild to severe iodine deficiency. In rats administered with these xenobiotics we measured a consistent increase in relative weight of the thyroids with increasing time and concentration of applied bromide, and a sharp reduction of the 24-h uptake of [I-131]-iodide by their thyroids. In these animals, we also determined a steady decline in serum total thyroxine concentration. At the molecular level, we found, unexpectedly, that the influence of exogenous bromide on the TPO enzyme activity in the rat thyroids was not simply inhibitory. It was more complex, biphasic with regard to the extent of bromide intake in the animals. With the use of several radioanalytical methods, including adapted radiometric determination of TPO enzyme activity, we therefore confirmed and quantified the presumed thyrotoxic effects of xenobiotics bromide and perchlorate ions.

Název v anglickém jazyce

RADIOMETRIC DETERMINATION OF THYROTOXIC EFFECTS OF SOME XENOBIOTICS

Popis výsledku anglicky

Recently, we have shown in the isolated rat thyroids marked effects of excessive exogenous bromide and perchlorate ions on the activity of thyroid peroxidase (TPO), the key enzyme in thyroid hormones (TH) metabolism. Here, we studied in more details, with the aid of several radioanalytical methods, effects of an enhanced bromide and/or perchlorate intake on various aspects of iodine metabolism and, consequently, on TH metabolism in the rat. Goitrogenic and thyrotoxic effects of excessive bromide and perchlorate ions were followed in adult male rats, maintained on diets with various iodine content, ensuring either sufficient iodine supply or mild to severe iodine deficiency. In rats administered with these xenobiotics we measured a consistent increase in relative weight of the thyroids with increasing time and concentration of applied bromide, and a sharp reduction of the 24-h uptake of [I-131]-iodide by their thyroids. In these animals, we also determined a steady decline in serum total thyroxine concentration. At the molecular level, we found, unexpectedly, that the influence of exogenous bromide on the TPO enzyme activity in the rat thyroids was not simply inhibitory. It was more complex, biphasic with regard to the extent of bromide intake in the animals. With the use of several radioanalytical methods, including adapted radiometric determination of TPO enzyme activity, we therefore confirmed and quantified the presumed thyrotoxic effects of xenobiotics bromide and perchlorate ions.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

20305 - Nuclear related engineering; (nuclear physics to be 1.3);

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

RAD 2015: THE THIRD INTERNATIONAL CONFERENCE ON RADIATION AND APPLICATIONS IN VARIOUS FIELDS OF RESEARCH

ISBN

9788680300016

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

463-466

Název nakladatele

RAD ASSOC

Místo vydání

Serbia

Místo konání akce

Budva, MONTENEGRO

Datum konání akce

8. 6. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000387979700096