Impact of Interspecific Hybridization of T. pratense x T. medium and Backcrossing on Genetic Variability of Progeny

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00087683" target="_blank" >RIV/00216224:14310/16:00087683 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.17221/115/2016-CJGPB" target="_blank" >http://dx.doi.org/10.17221/115/2016-CJGPB</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/115/2016-CJGPB" target="_blank" >10.17221/115/2016-CJGPB</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Impact of Interspecific Hybridization of T. pratense x T. medium and Backcrossing on Genetic Variability of Progeny

Popis výsledku v původním jazyce

Red clover (Trifolium pratense L.) is a high-quality fodder crop which has been hybridized successfully with its wild relative zigzag clover (T. medium L.). The aim of this study was to evaluate the genetic impact of interspecific hybridization and subsequent repeated backcrossing on the variability within hybrid progeny genomes. Nuclear DNA content of 800 and 753 hybrid plants from F-7/F-8 and F-8/F-9 generations, respectively, was measured by flow cytometry. Resulting values were converted to estimated chromosome counts, which were successfully validated on a sample of 28 plants by counting mitotic chromosomes. The two generations showed a similar distribution of various chromosome counts ranging from 22 to 47 chromosomes. In total, 24.0% and 34.3% of plants from the two generations had different numbers of chromosomes from their parental plants. Variability within the hybrid population was assessed by fluorescent in situ hybridization using rDNA probes.

Název v anglickém jazyce

Impact of Interspecific Hybridization of T. pratense x T. medium and Backcrossing on Genetic Variability of Progeny

Popis výsledku anglicky

Red clover (Trifolium pratense L.) is a high-quality fodder crop which has been hybridized successfully with its wild relative zigzag clover (T. medium L.). The aim of this study was to evaluate the genetic impact of interspecific hybridization and subsequent repeated backcrossing on the variability within hybrid progeny genomes. Nuclear DNA content of 800 and 753 hybrid plants from F-7/F-8 and F-8/F-9 generations, respectively, was measured by flow cytometry. Resulting values were converted to estimated chromosome counts, which were successfully validated on a sample of 28 plants by counting mitotic chromosomes. The two generations showed a similar distribution of various chromosome counts ranging from 22 to 47 chromosomes. In total, 24.0% and 34.3% of plants from the two generations had different numbers of chromosomes from their parental plants. Variability within the hybrid population was assessed by fluorescent in situ hybridization using rDNA probes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

<a href="/cs/project/QI111A019" target="_blank" >QI111A019: Nové genomické postupy pro šlechtění cizosprašných plodin na zlepšení užitkových vlastností</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Genetics and Plant Breeding

ISSN

1212-1975

e-ISSN

—

Svazek periodika

52

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

125-131

Kód UT WoS článku

000390388200001

EID výsledku v databázi Scopus

2-s2.0-85006337230